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Instructions and questions for the first exam of isat 454, a university course likely focused on protein analysis and visualization. Students are required to identify proteins, create gif files using rasmol, write captions, and analyze metal ions and mutations in protein structures. References are needed for each answer.
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_Your name:_______________________________ 25 points total
Go to the pdb main page and download, 1 IRL and 3INK a) (2 points) What is the name of this protein? ________________What is its function? b) (4 points) Use RasMol to create two side by side gif files that illustrate the difference between these two structures. The gif files should have a white background and shows the protein as ribbons, colored by “structure.” Any individual amino acids that you might want to highlight should be in red spacefill. Paste your gif files in the text boxs below. c) (4 points) Write a good caption for this these figures. d) (2 points) There is a disulfide bond in the 1IRL structure. Which specific amino acids are involved in this disulfide bond (give the name of the type of amino acid and the amino acid number for each.)