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Fungal Biology: Characteristics, Reproduction, Diseases, and Economic Importance, Lecture notes of Microbiology

A comprehensive overview of fungal biology, covering their characteristics, reproduction, diseases, and economic importance. It delves into the structure of fungi, including hyphae and reproductive structures, and explores both asexual and sexual reproduction. The document also discusses various fungal diseases, including mycoses, allergies, and toxins, and highlights the economic significance of fungi in industries like fermentation and research. It further explores the symbiotic relationship between fungi and algae in lichens.

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2024/2025

Uploaded on 02/25/2025

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Part I: Fungal Characteristics
Fungi
Fungi are chemoheterotrophs, meaning:
Chemo-: Their energy comes from chemicals.
Heterotroph: Their carbon comes from organic compounds, like decomposing
organic matter.
Types of Fungi:
Molds: Multicellular
Yeasts: Unicellular
Fungi are crucial in ecosystems because they break down organic matter (e.g., fallen
trees) and recycle carbon (C) and nitrogen (N).
Key Characteristics of Fungi:
1. Aerobic: They require oxygen for growth, though some are facultative anaerobes
(can grow with or without oxygen).
2. Decomposers: They help cycle carbon and nitrogen back into the environment.
3. Cell Wall: Some fungi have cell walls made of chitin, the same material found in
the exoskeletons of insects and crustaceans.
4. High Tolerance: Fungi can grow in high concentrations of sugar, salt, and acid,
which is why they often appear on spoiled food before bacteria do.
Structure of Fungi
1. Hyphae: Filaments that make up the body of the fungus.
oSeptate Hyphae: Hyphae with cross-walls dividing the cells.
oAseptate Hyphae: Hyphae without cross-walls (also called cenocytic
hyphae).
2. Mycelium: The mass of hyphae, often found underground or in the substance the
fungus is growing on (like bread or potatoes).
3. Reproductive Structures:
oAerial Hyphae (Reproductive Hyphae): Hold the spores and are often
visible above the surface.
oVegetative Hyphae: Involved in nutrient absorption but don't produce
spores.
oRhizoidal Hyphae: Hyphae that penetrate into the substrate to obtain
nutrients.
4. Dimorphism: Some fungi can switch between yeast-like and mold-like growth,
depending on environmental factors like temperature.
Reproduction of Fungi
Fungi reproduce both sexually and asexually.
Asexual Reproduction:
oInvolves the formation of spores that are clones of the parent fungus.
oSpores travel to new areas, grow into hyphae, and form a new mycelium.
Sexual Reproduction:
oInvolves genetic recombination between two parent fungi (donor and
recipient).
oThe key terms for understanding sexual reproduction in fungi are:
1. Plasmogamy: The fusion of two haploid cells (one set of
chromosomes from each parent).
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Part I: Fungal Characteristics

Fungi Fungi are chemoheterotrophs, meaning:  Chemo- : Their energy comes from chemicals.  Heterotroph : Their carbon comes from organic compounds, like decomposing organic matter. Types of Fungi :  Molds : Multicellular  Yeasts : Unicellular Fungi are crucial in ecosystems because they break down organic matter (e.g., fallen trees) and recycle carbon (C) and nitrogen (N). Key Characteristics of Fungi :

  1. Aerobic : They require oxygen for growth, though some are facultative anaerobes (can grow with or without oxygen).
  2. Decomposers : They help cycle carbon and nitrogen back into the environment.
  3. Cell Wall : Some fungi have cell walls made of chitin, the same material found in the exoskeletons of insects and crustaceans.
  4. High Tolerance : Fungi can grow in high concentrations of sugar, salt, and acid, which is why they often appear on spoiled food before bacteria do. Structure of Fungi
  5. Hyphae : Filaments that make up the body of the fungus. o Septate Hyphae : Hyphae with cross-walls dividing the cells. o Aseptate Hyphae : Hyphae without cross-walls (also called cenocytic hyphae).
  6. Mycelium : The mass of hyphae, often found underground or in the substance the fungus is growing on (like bread or potatoes).
  7. Reproductive Structures : o Aerial Hyphae (Reproductive Hyphae) : Hold the spores and are often visible above the surface. o Vegetative Hyphae : Involved in nutrient absorption but don't produce spores. o Rhizoidal Hyphae : Hyphae that penetrate into the substrate to obtain nutrients.
  8. Dimorphism : Some fungi can switch between yeast-like and mold-like growth, depending on environmental factors like temperature. Reproduction of Fungi Fungi reproduce both sexually and asexually.  Asexual Reproduction : o Involves the formation of spores that are clones of the parent fungus. o Spores travel to new areas, grow into hyphae, and form a new mycelium.  Sexual Reproduction : o Involves genetic recombination between two parent fungi (donor and recipient). o The key terms for understanding sexual reproduction in fungi are:
  9. Plasmogamy : The fusion of two haploid cells (one set of chromosomes from each parent).
  1. Karyogamy : The fusion of the nuclei of the two cells, creating a diploid nucleus.
  2. Meiosis : The process that reduces the diploid cell back to haploid, producing genetically diverse spores. Life Cycles of Fungi Fungi can reproduce sexually and asexually, and the life cycle varies by group. Examples from different fungal divisions are discussed:
  3. Zygomycota (e.g., Rhizopus) : o Asexual : Spores produced by aerial hyphae. o Sexual : Fusion of two haploid cells (plasmogamy) followed by nuclear fusion (karyogamy) and the formation of genetically diverse spores through meiosis.
  4. Ascomycota : o Asexual : Similar to zygomycota, with spores formed asexually. o Sexual : Includes plasmogamy, karyogamy, and meiosis to form genetically diverse spores.
  5. Basidiomycota (e.g., mushrooms) : o Asexual : Spores are produced by specialized structures. o Sexual : Involves plasmogamy, karyogamy, and meiosis to produce spores. Summary of Key Terms in Fungal ReproductionPlasmogamy : Fusion of two haploid cells.  Karyogamy : Fusion of the haploid nuclei to form a diploid nucleus.  Meiosis : Division process that produces genetically diverse spores.

Part II: Fungal Diseases

Practical Tips for Understanding Fungi  The terms can be overwhelming, but focusing on these key concepts will help you understand fungal biology: o Hyphae : Filaments that make up the fungal body. o Mycelium : A network of hyphae. o Spores : Reproductive units, either sexually or asexually produced. o Dimorphism : Ability of some fungi to change growth forms. Fungal Allergies and Toxins:Fungal Spores and Allergies : Some people are allergic to fungal spores, leading to respiratory issues. The "mold count" in the news reports how many spores are in the air.  Fungal Toxins : Some fungi produce toxins, which can be harmful:

  1. Ergot Poisoning : Caused by fungi that grow on rye and wheat during poor storage. Historically, it was linked to the Salem witch trials, where children displayed symptoms like hallucinations. Ergot contains lysergic acid , a compound similar to LSD.
  2. Aflatoxin : Produced by the fungus Aspergillus flavus , aflatoxin can be carcinogenic. It is often associated with improperly stored peanuts and has caused scares, but no significant threat was found.

o Genetic & Biochemical Tools: Fungi are used in research due to their eukaryotic nature.

  1. Fungal Diseases in Plants : o Example: Oak wilt in Texas affects oak trees and can spread through pruning or insect vectors. Lichens:Lichens : A symbiotic relationship between fungi and algae. Fungi provide a habitat, while algae perform photosynthesis and provide food.  Air Quality Indicator : Lichens grow only in clean air environments.  Pioneer Colonizers : Lichens are among the first organisms to colonize devastated areas.

Part III: Algae and Protozoa

Algae OverviewEukaryotic organisms : Can be unicellular or multicellular.  Photosynthetic : Algae are photoautotrophs, meaning they use light for energy and carbon dioxide (CO2) for carbon.  Color and Pigment : Algae are categorized based on their pigments, which allow them to capture light energy. Common pigments include: o Chlorophyll (for photosynthesis). o Accessory pigments : These give algae their unique color (carotene, xanthophylls). Types of Algae

  1. Brown Algae : Example - Sargassum (found in the Gulf of Mexico).
  2. Green Algae : Often have air bladders (which help keep blades out of the water).
  3. Red Algae : Typically found deeper in the water, as they capture different wavelengths of light. Algal StructureThallus : The whole body of the algae (no true roots, stems, or leaves).  Holdfast : Anchors algae to surfaces.  Stipe : Similar to a stem.  Blades : Leaf-like structures, responsible for capturing light energy.  Air Bladders : Help keep blades splayed out for optimal light capture. Algal Habitats  Algae can be found in both marine and freshwater environments.  They do not have specialized tissues like xylem and phloem (found in plants), so nutrients and water are absorbed directly through the body. Role in EcosystemsPrimary producers : Form the foundation of food webs in aquatic environments by converting CO2 into organic molecules.  Oxygen production : Algae are responsible for a significant portion of oxygen production, especially in aquatic systems.  Planktonic Algae : These free-floating organisms contribute to the plankton in oceans. Algal BloomsAlgal blooms : Rapid growth of algae due to an influx of nutrients (e.g., nitrogen and phosphorus from rain).

Negative impacts : When algae die, they consume oxygen in the water, leading to hypoxia and death of marine life. Algae Classification by Pigment  Algae are classified by their pigment types (green, red, brown, gold).  Green algae have chlorophyll, while other types have accessory pigments like carotene and xanthophylls. Special ApplicationsAgar : Derived from red algae and used as a gelling agent in laboratories. Protozoa OverviewUnicellular eukaryotes : Unlike algae, protozoa are always unicellular and do not have chlorophyll.  Chemoheterotrophs : Obtain energy and carbon from organic chemicals (similar to fungi and humans).  Movement : Protozoa are classified based on their mode of movement (e.g., flagella, cilia, pseudopodia). Protozoa ReproductionSchizogony : A type of asexual reproduction where the nucleus undergoes multiple divisions before the cell divides into smaller cells. This allows protozoa to reproduce quickly and reach large numbers. Protozoa Diseases  Protozoa can cause various diseases, including: o Malaria (Plasmodium species) o Sleeping sickness (Trypanosoma) o Toxoplasmosis (caused by Toxoplasma, associated with cats) o Vaginitis (can be caused by protozoa like Trichomonas) Protozoa and Plankton  Protozoa are part of the plankton, feeding on bacteria and other small organisms in the ocean. DinoflagellatesUnicellular protozoa that contribute to plankton.  Some dinoflagellates are toxic and can produce neurotoxins harmful to aquatic life and humans.  Examples : o Gymnodinium : Toxin harms fish by blocking gill function. o Alexandrium : Produces toxins that can cause paralytic shellfish poisoning (PSP) in humans, with symptoms ranging from dizziness to death if enough toxin is ingested. Summary  Algae: Can be unicellular or multicellular, use light for energy, and are classified by pigment type.  Protozoa: Always unicellular, chemoheterotrophs, and classified by movement, with some causing diseases.  Part IV: Protozoal Diseases

1. ToxoplasmosisCausative Agent : Toxoplasma gondii (protozoan)

Risk : The disease was originally common in Central and South America but has now spread to southern parts of the U.S., including Texas, likely due to the movement of people and animals.  Prevention : Eliminating kissing bugs from the environment by sealing cracks in homes and using insect repellents. Summary of Protozoa Characteristics:Chemoheterotrophic : All the protozoa discussed are chemoheterotrophs, meaning they absorb nutrients from their environment.  Unicellular : These organisms are single-celled.  Movement : Protozoa move in various ways: some use cilia, others flagella, and some use amoeboid movement.  Cysts : Some protozoa can form cysts, which allow them to survive in harsh environmental conditions and during transmission. Key Points to Remember:Transmission Routes : Many protozoan infections spread via the fecal-oral route, often through contaminated water or direct contact with infected material.  Water Contamination : Protozoa like Cryptosporidium and Giardia are resistant to chlorine, making water filtration an essential preventive measure.  Insect Vectors : Infections like Chagas and Leishmaniasis are transmitted by insects (kissing bugs and sandflies).  Impact on Pregnancy : Toxoplasmosis poses a significant risk to pregnant women, so extra care is needed in preventing exposure.  Part V: Helminths Chapter 12: Helmets and Vectors Introduction to Helmets (Worms)Helmets refer to worms, which are multicellular organisms that have complex systems like a digestive tract and circulatory system.  We focus on three main groups:

  1. Nematodes (Roundworms)
  2. Cestodes (Tapeworms)
  3. Trematodes (Flukes) – Not covered in this course due to their complexity. Nematodes (Roundworms)Pinworms : o Common in children, often contracted through contaminated toys or dirt. o Symptoms: Itching around the anus, most noticeable at night when pinworms come out of the anus. o Diagnosis: Tape test (stick tape to the anus at night and check in the morning for worms). o Treatment: A single pill for the entire family, as it’s highly contagious. o Heartworms : Found in dogs and cats, contracted through fleas. Cestodes (Tapeworms)Transmission : Consuming undercooked meat.  Structure : Segmented bodies with a specialized head (scolex) that attaches to the intestinal wall using hooks and suckers.

Diagnosis and Treatment : The tapeworm can grow many feet long, but treatment involves targeting the head to prevent further growth. Cooking meat thoroughly helps prevent infection.  Transmission in Animals : Pets like cats and dogs often get tapeworms from ingesting fleas. Trematodes (Flukes)  Not discussed in detail, as they are too complex for the course material. VectorsDefinition : A vector is an organism that carries and transmits a disease-causing pathogen.  Insects as Vectors : Insects fall under the group arthropods , and they play a key role in transmitting diseases. Examples of Vectors:

  1. Mosquitoes : o Female mosquitoes transmit diseases like malaria through their blood meal. o They have a proboscis (mouthpiece) that helps them draw blood. o Different species of mosquitoes carry different pathogens.
  2. Fleas : o Vectors for the bubonic plague. o They are commonly found on pets and other animals.
  3. Lice : o Can carry various pathogens, causing diseases such as typhus. o Lice can also infest the genital area, leading to pubic lice (also known as crabs), a sexually transmitted infection.
  4. Ticks : o Vectors for diseases like Rocky Mountain Spotted Fever and Lyme Disease. o They are more thoroughly discussed in the bacteria chapter.
  5. Kissing Bugs : o Transmit Chagas disease.