Docsity
Log inSign up
Docsity
Prepare for your exams
Prepare for your exams

Study with the several resources on Docsity

Earn points to download
Earn points to download

Earn points by helping other students or get them with a premium plan

Guidelines and tips
Guidelines and tips
Sell on Docsity
Sell on Docsity
Log inSign up

Prepare for your exams

Study with the several resources on Docsity

Find documents

Prepare for your exams with the study notes shared by other students like you on Docsity

Search Store documents

The best documents sold by students who completed their studies

Search through all study resources
Docsity AINEW

Summarize your documents, ask them questions, convert them into quizzes and concept maps

Explore questions

Clear up your doubts by reading the answers to questions asked by your fellow students

Earn points to download

Earn points by helping other students or get them with a premium plan

Share documents
download point icon20 Points

For each uploaded document

Answer questions
download point icon5 Points

For each given answer (max 1 per day)

All the ways to get free points
Get points immediately

Choose a premium plan with all the points you need

Study Opportunities

Choose your next study program

Get in touch with the best universities in the world. Search through thousands of universities and official partners

Community

Ask the community

Ask the community for help and clear up your study doubts

University Rankings

Discover the best universities in your country according to Docsity users

Free resources

Our save-the-student-ebooks!

Download our free guides on studying techniques, anxiety management strategies, and thesis advice from Docsity tutors

From our blog

Exams and Study
Go to the blog

Cell Biology - Examination 2 with Answers | BIOL 4374, Exams of Cell Biology

University of Houston (UH)Cell Biology

Material Type: Exam; Class: Cell Biology; Subject: (Biology); University: University of Houston; Term: Spring 2014;

Typology: Exams

2013/2014

Uploaded on 05/10/2014

unknown user
unknown user 🇺🇸

1 / 10

Toggle sidebar

This page cannot be seen from the preview

Don't miss anything!

bg1
Cell$Biology/Cell$Biochemistry:$Exam%#2%
Choose&the&single&best&answer&
!
1)! A!protein!that!facilitates!the!correct!folding!or!assembly!of!other!proteins!is!called!a(n)!
________________________.!
!
A.! Elongation!factor!
B.! Ribosome!
C.! Telomerase!
D.!! Slide!clamp!
E.!Chaperone!
!
2)! !A!large!protease!complex!that!degrades!proteins!tagged!by!ubiquitin!is!called!__________________.!
!
A.! Proteosome!
B.! Hydrosome!
C.!Chaperone!
D.!! Lysosome!
E.! Autophagosome!
!
3)!“Gene!expression”!refers!to____________________?!
!
A.! DNAàRNA!
B.!DNAàRNAàprotein!
C.! All!of!the!above!
!
4)! Double!strand!DNA!breaks!can!be!repaired!flawlessly!by:!
!
A. the!DNA!mismatch!repair!system.!
B. Homologous!recombination!
C. Nonhomologous!end!joining.!
D. DNA!maintenance!methyltranferase.!
!
5)! You!have!a!piece!of!DNA!that!includes!the!following!sequence:!
!
5′WATAGGCATTCGATCCGGATAGCATW3′!
3′WTATCCGTAAGCTAGGCCTATCGTAW5′!
!
Which!of!the!following!RNA!molecules!could!be!transcribed!from!this!piece!of!DNA?!
!
A.! 5′WUAUCCGUAAGCUAGGCCUAUGCUAW3′!
B.!5′WAUAGGCAUUCGAUCCGGAUAGCAUW3′!
C.!5′WUACGAUAGGCCUAGCUUACGGAUAW3′!
D.!none!of!the!above!
!
!
!
!
!
pf3
pf4
pf5
pf8
pf9
pfa

Partial preview of the text

Download Cell Biology - Examination 2 with Answers | BIOL 4374 and more Exams Cell Biology in PDF only on Docsity!

Cell Biology/Cell Biochemistry: Exam

Choose the single best answer

  1. A protein that facilitates the correct folding or assembly of other proteins is called a(n) ________________________. A. Elongation factor B. Ribosome C. Telomerase D. Slide clamp E. Chaperone
  2. A large protease complex that degrades proteins tagged by ubiquitin is called __________________. A. Proteosome B. Hydrosome C. Chaperone D. Lysosome E. Autophagosome
  3. “Gene expression” refers to____________________? A. DNAàRNA B. DNAàRNAàprotein C. All of the above 4 ) Double strand DNA breaks can be repaired flawlessly by: A. the DNA mismatch repair system. B. Homologous recombination C. Nonhomologous end joining. D. DNA maintenance methyltranferase.
  4. You have a piece of DNA that includes the following sequence: 5′-­‐ATAGGCATTCGATCCGGATAGCAT-­‐3′ 3′-­‐TATCCGTAAGCTAGGCCTATCGTA-­‐5′ Which of the following RNA molecules could be transcribed from this piece of DNA? A. 5′-­‐UAUCCGUAAGCUAGGCCUAUGCUA-­‐3′ B. 5′-­‐AUAGGCAUUCGAUCCGGAUAGCAU-­‐3′ C. 5′-­‐UACGAUAGGCCUAGCUUACGGAUA-­‐3′ D. none of the above
  1. In a gel filtration column: A. Smaller proteins enter the beads more readily. B. Large proteins elute first. C. Small proteins elute first. D. Large proteins enter the beads more readily. E. both A and B
  2. Total nucleic acids are extracted from a culture of yeast cells and are then mixed with resin beads to which the polynucleotide 5′-­‐TTTTTTTTTTTTTTTTTTTTTTTTT-­‐ 3 ′ has been covalently attached. After a short incubation, the beads are then extracted from the mixture. When you analyze the cellular nucleic acids that have stuck to the beads, which of the following is most abundant? A. DNA B. tRNA C. rRNA D. mRNA
  3. Which of the following technologies allows us to study the expression patterns of thousands of genes at the same time? A. Southern Blotting B. DNA microarrays C. RNA-­‐Seq D. In situ hybridization E. B and C
  4. The figure below depicts the restriction map of one segment of the human genome for four restriction nucleases W, X, Y, and Z. The figure below depicts the restriction maps of four individual BAC clones that contain segments of human DNA from the region depicted in the figure below. From this information, how would you order these BAC clones, from left to right? A. 1, 2, 3, 4 B. 2, 1, 4, 3 C. 3, 4, 2, 1 D. 4, 1, 3, 2 W X Y W W Z X Y Z (A) (B) BAC# BAC# BAC# BAC#
  1. You are studying an enzyme in which an active site cysteine residue is encoded by the triplet UGU. How would mutating the third base to a C affect the enzyme? A. It would have no impact. B. It would result in a frameshift mutation. C. It would result in a cysteine-­‐to-­‐alanine mutation. D. It would lead to a truncated protein. 15 ) Which of the following statements is true? A. Ribosomes are large RNA structures composed solely of rRNA. B. Ribosomes are synthesized entirely in the cytoplasm. C. rRNA contains the catalytic activity that joins amino acids together. D. A ribosome binds one tRNA at a time. 16 ) The figure below shows the cleavage site of several restriction nucleases. You cut a vector using the PclI restriction nuclease. Which of the following restriction nucleases will generate a fragment that can be ligated into this cut vector with the addition of only ligase and ATP? A. HindIII B. NcoI C. MmeI D. NspV 17 ) In humans and in chimpanzees, 99% of the Alu retrotransposons are in corresponding positions. Which of the following statements below is the most likely explanation for this similarity? A. The Alu retrotransposon is not capable of transposition in humans. B. Most of the Alu sequences in the chimpanzee genome underwent duplication and divergence before humans and chimpanzees diverged. C. The Alu retrotransposons are in the most beneficial position in the genome for primates. D. The Alu retrotransposons must also be in the same position in flies. 18 ) In sexually reproducing organisms, only changes to the germ line are passed along to progeny. A. True B. False 5 ¢ 3 ¢ 3 ¢ 5 ¢ A C A T G T T G T A C A PclI 5 ¢ 3 ¢ 3 ¢ 5 ¢ A A G C T T T T C G A A HindIII 5 ¢ 3 ¢ 3 ¢ 5 ¢ C C A T G G G G T A C C NcoI 5 ¢ 3 ¢ 3 ¢ 5 ¢ C C A T G G G G T A C C MmeI 5 ¢ 3 ¢ 3 ¢ 5 ¢ A C A T G T T G T A C A NspV

19 ) You have a piece of circular DNA that can be cut by the restriction nucleases XhoI and SmaI, as indicated in the figure below. If you were to cut this circular piece of DNA with both XhoI and SmaI, how many fragments of DNA would you end up with? A. 1 B. 2 C. 3 D. 4 20 ) An enzyme that phosphorylates proteins by transferring a phosphate group from ATP is called a(n) ______________________. A. Transferase B. Phosphatase C. Histone D. Kinase E. Dehydrogenase 21 ) In Southern blotting DNA fragments are first separated from each other by: A. electroelution. B. gel electrophoresis. C. column chromatography. D. centrifugation. 22 ) pBR322 (a plasmid vector) has a single BamHI site and a single BspMI site. A double digestion produces two fragments of approximately 690 bp and 3670 bp. Map the two sites on the circular plasmid. If the BamHI site is at the beginning of the plasmid tetracycline resistance gene and the gene is about 1200 bp long, is the BspMI site also in the gene? A. Yes B. You can’t determine the answer from the data C. No 23 ) Which DNA sequence is most likely part of an origin of replication? A. ATGCATGCATGCATGC B. GCATGCATGCATATGC C. GCATGCGGGCCATGCT D. TATTACTAAATTAATT Xhol Smal

29 ) You grow E. coli for several generations in media containing 15 N. The cells are then transferred to media containing 14 N and grown for two additional generations. What proportion of dsDNA isolated from these cells will be heavy, light or intermediate in density? A. Heavy = 100%; Light = 0%; Intermediate = 0% B. Heavy = 0%; Light = 100%; Intermediate = 0% C. Heavy = 50%; Light = 0%; Intermediate = 50% D. Heavy = 33%; Light = 33%; Intermediate = 33% E. Heavy = 0%; Light = 50%; Intermediate = 50% 30 ) Patients with xeroderma pigmentosum suffer an extremely high incidence of skin cancer but have not been found to have correspondingly high incidences of cancers of internal organs (e.g. colon cancer) nor are disease rates altered in specific families? What types of cells are most likely mutated xeroderma pigmentosum? A. Somatic B. Germline 31 ) Where does translation occur? A B

C

NOTE: Use the below image, derived from prokaryotes, for questions 32-­‐ 34.

  1. What is “2” referring to? A. start codon B. stop codon C. 5’ untranslated region D. 3’ untranslated region E. protein-­‐coding region
  2. What is “6” referring to? A. start codon B. stop codon C. 5’ untranslated region D. 3’ untranslated region E. protein-­‐coding region 34 ) What is “5” referring to? A. start codon B. stop codon C. 5’ untranslated region D. 3’ untranslated region E. protein-­‐coding region 35 ) RNAi is triggered by the presence of foreign __________________ molecules, which are digested by the __________________ enzyme into shorter fragments approximately 23 nucleotide pairs in length. A. double-­‐stranded RNA, dicer B. tRNA, dicer C. RISC, mRNA D. mitochondria, single-­‐stranded RNA E. proteasome, autophagosomes 36 ) DNA can be introduced into bacteria by a mechanism called ____________. A. transcription B. ligation C. replication D. transformation

NOTE: Use the information below for questions 40-­‐ 42. You have an oligonucleotide probe that hybridizes to part of gene A from a eucaryotic cell. Indicate whether a cDNA library or a genomic DNA library will be more appropriate for use in the following applications.

  1. You want to study the promoter of a gene A. A. cDNA library B. genomic library
  2. Gene A encodes a tRNA and you wish to isolate a piece of DNA containing the full-­‐length sequence of the tRNA. A. cDNA library B. genomic library
  3. You want to express gene A in bacteria to produce lots of protein A. A. cDNA library B. genomic library
  4. Which noncoding RNAs are involved in alternative splicing? A. miRNAs B. rRNAs C. tRNAs D. snRNAs E. mRNAs
  5. Which of the following is not a general mechanism that cells use to maintain stable patterns of gene expression as cells divide? A. a positive feedback loop, mediated by a transcriptional regulator that activates transcription of its own gene in addition to other cell-­‐type specific genes B. faithful propagation of condensed chromatin structures as cells divide C. inheritance of DNA methylation patterns when cells divide D. proper segregation of housekeeping proteins when cells divide
  6. You want to amplify the DNA between the two stretches of sequence shown in the figure below. Of the listed primers, choose the pair that will allow you to amplify the DNA by PCR. A. 1 and 8 B. 2 and 6 C. 3 and 4 D. 4 and 5 E 2 and 8