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Material Type: Exam; Class: Cell Biology; Subject: (Biology); University: University of Houston; Term: Spring 2014;
Typology: Exams
Uploaded on 05/10/2014
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19 ) You have a piece of circular DNA that can be cut by the restriction nucleases XhoI and SmaI, as indicated in the figure below. If you were to cut this circular piece of DNA with both XhoI and SmaI, how many fragments of DNA would you end up with? A. 1 B. 2 C. 3 D. 4 20 ) An enzyme that phosphorylates proteins by transferring a phosphate group from ATP is called a(n) ______________________. A. Transferase B. Phosphatase C. Histone D. Kinase E. Dehydrogenase 21 ) In Southern blotting DNA fragments are first separated from each other by: A. electroelution. B. gel electrophoresis. C. column chromatography. D. centrifugation. 22 ) pBR322 (a plasmid vector) has a single BamHI site and a single BspMI site. A double digestion produces two fragments of approximately 690 bp and 3670 bp. Map the two sites on the circular plasmid. If the BamHI site is at the beginning of the plasmid tetracycline resistance gene and the gene is about 1200 bp long, is the BspMI site also in the gene? A. Yes B. You can’t determine the answer from the data C. No 23 ) Which DNA sequence is most likely part of an origin of replication? A. ATGCATGCATGCATGC B. GCATGCATGCATATGC C. GCATGCGGGCCATGCT D. TATTACTAAATTAATT Xhol Smal
29 ) You grow E. coli for several generations in media containing 15 N. The cells are then transferred to media containing 14 N and grown for two additional generations. What proportion of dsDNA isolated from these cells will be heavy, light or intermediate in density? A. Heavy = 100%; Light = 0%; Intermediate = 0% B. Heavy = 0%; Light = 100%; Intermediate = 0% C. Heavy = 50%; Light = 0%; Intermediate = 50% D. Heavy = 33%; Light = 33%; Intermediate = 33% E. Heavy = 0%; Light = 50%; Intermediate = 50% 30 ) Patients with xeroderma pigmentosum suffer an extremely high incidence of skin cancer but have not been found to have correspondingly high incidences of cancers of internal organs (e.g. colon cancer) nor are disease rates altered in specific families? What types of cells are most likely mutated xeroderma pigmentosum? A. Somatic B. Germline 31 ) Where does translation occur? A B
NOTE: Use the below image, derived from prokaryotes, for questions 32-‐ 34.
NOTE: Use the information below for questions 40-‐ 42. You have an oligonucleotide probe that hybridizes to part of gene A from a eucaryotic cell. Indicate whether a cDNA library or a genomic DNA library will be more appropriate for use in the following applications.