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Catalase Lab Manual, Lab Reports of Biology

Catalase enzyme which catalyzes the decomposition of hydrogen peroxide to water and oxygen.

Typology: Lab Reports

2020/2021

Uploaded on 05/11/2021

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NAME
PERIOD
DATE
CATALASE LAB
Introduction
In this investigation, you will study several factors that affect the activity of enzymes.
The enzyme to be used is catalase (also called peroxidase), present in most cells and found in
high concentrations in liver and blood cells. You will use liver homogenate as the source of
catalase. Catalase promotes the decomposition of hydrogen peroxide, H2O2 in the following
reaction.
2H2O2 2H2O + O2
Hydrogen peroxide is formed as a by-product of chemical reactions in cells. It is toxic and
would soon kill cells if not immediately removed or broken down.
Materials
10 mL graduated cylinder
100 mL graduated cylinder
reaction chamber (dropper bottle with cut off dropper)
pan of water (enough to cover 100 mL graduated cylinder when lying on side)
hydrogen peroxide
catalase solution
timer
pf3
pf4

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NAME PERIOD DATE

CATALASE LAB

Introduction

In this investigation, you will study several factors that affect the activity of enzymes. The enzyme to be used is catalase (also called peroxidase), present in most cells and found in high concentrations in liver and blood cells. You will use liver homogenate as the source of catalase. Catalase promotes the decomposition of hydrogen peroxide, H 2 O 2 in the following reaction. 2H 2 O 2 ◊ 2H 2 O + O 2 Hydrogen peroxide is formed as a by-product of chemical reactions in cells. It is toxic and would soon kill cells if not immediately removed or broken down.

Materials

10 mL graduated cylinder 100 mL graduated cylinder reaction chamber (dropper bottle with cut off dropper) pan of water (enough to cover 100 mL graduated cylinder when lying on side) hydrogen peroxide catalase solution timer

Procedure

NOTE: In all experiments, make certain that your reaction chamber is extremely clean. Catalase is a potent enzyme, and if the chamber is not washed thoroughly, enough will adhere to the sides to make subsequent tests inaccurate.

  1. Obtain all materials. Be sure pan is filled with water.
  2. Stand the reaction chamber upright and carefully add 10 mL of hydrogen peroxide solution.
  3. Tightly stopper the chamber to avoid evaporation.
  4. Lay the 100 mL graduated cylinder on its side in the pan so that it fills with water completely. If any air bubbles are present, carefully work these out by tilting the cylinder slightly while keeping it underwater at all times. The next steps need to be performed quickly but safely in order to produce accurate results. The goal is to capture the air bubbles produced from the reaction chamber into the graduated cylinder as early as possible after the reaction begins.
  5. Add three drops of catalase to the hydrogen peroxide in the reaction chamber, stopper the chamber and immediately submerge the chamber in your pan of water. Be sure to orient the tip of the chamber is beneath the graduated cylinder allowing the air bubbles to move directly from the reaction chamber to the graduated cylinder.
  6. Every 30 seconds, measure and record the amount of air in the graduated cylinder. Take measurements for 10 minutes for each trial.
  7. Thoroughly clean your reaction chamber and repeat the same procedure using 1 drop of enzyme for trial 2, 2 drops of enzyme for trial 3 and 4 drops of enzyme for trial 4. Data Table - rough draft mL of Gas Collected drops of catalase
  1. If you had allowed the trial using 1 drop to continue past 10 minutes until the reaction had run to completion, approximately how much O 2 do you think would be produced? (1 point) Fully explain and support your answer. Refer to data and/or graph or class discussion when necessary. (2 points)
  2. What do you think would happen to the amount of O 2 produced if you ran the experiment in a pan of hot water? (1 point) Fully explain and support your answer. Refer to data and/or graph or class discussion when necessary. (2 points)
  3. What do you think would happen to the amount of O 2 produced if you added 20 mL of hydrogen peroxide to your reaction chamber instead of 10 mL? (1 point) Fully explain and support your answer. Refer to data and/or graph or class discussion when necessary. (2 points)