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Catalase enzyme which catalyzes the decomposition of hydrogen peroxide to water and oxygen.
Typology: Lab Reports
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In this investigation, you will study several factors that affect the activity of enzymes. The enzyme to be used is catalase (also called peroxidase), present in most cells and found in high concentrations in liver and blood cells. You will use liver homogenate as the source of catalase. Catalase promotes the decomposition of hydrogen peroxide, H 2 O 2 in the following reaction. 2H 2 O 2 ◊ 2H 2 O + O 2 Hydrogen peroxide is formed as a by-product of chemical reactions in cells. It is toxic and would soon kill cells if not immediately removed or broken down.
10 mL graduated cylinder 100 mL graduated cylinder reaction chamber (dropper bottle with cut off dropper) pan of water (enough to cover 100 mL graduated cylinder when lying on side) hydrogen peroxide catalase solution timer
NOTE: In all experiments, make certain that your reaction chamber is extremely clean. Catalase is a potent enzyme, and if the chamber is not washed thoroughly, enough will adhere to the sides to make subsequent tests inaccurate.