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A laboratory procedure for measuring the ph changes in deionized water and acetate buffers after adding hydrochloric acid (hcl) and sodium hydroxide (naoh). The experiment involves preparing the buffers, measuring their initial ph, and then adding acid or base dropwise while recording the ph at each interval until a significant change is observed. The document also includes instructions for preparing sorensen's phosphate buffer and determining its buffer capacity.
Typology: Lab Reports
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Solution Calculated pH Observed pH DI water DI water + 1 drop 0.1 M HCl DI water + 10 drops 0.1 M HCl DI water + 1 drop 0.1 M NaOH DI water + 10 drops 0.1 M NaOH
pH of acetic acid: pH of acetate:
Buffer ObservedpH
Predicted Resistance: More to acid, base, or the same?
pH after 1 drop 0. M HCl
pH after 10 drops 0. M HCl
TOTAL volume HCl to change pH by one unit
TOTAL volume HCl to change pH by two units
TOTAL volume HCl to change pH by three units A B C
Buffer ObservedpH
Predicted Resistance: More to acid, base, or the same?
pH after 1 drop 0. M NaOH
pH after 10 drops 0. M NaOH
TOTAL volume NaOH to change pH by one unit
TOTAL volume NaOH to change pH by two units
TOTAL volume to change pH by four units A B C
Diluting a buffer:
pH of buffer before dilution: pH of buffer after dilution: