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BrdU Cell Proliferation Assay: A Method to Quantify Cell Proliferation, Essays (university) of Health sciences

The brdu cell proliferation assay is a versatile and convenient method to quantify cell proliferation by labeling nascent dna in actively dividing cells using bromodeoxyuridine (brdu). The intensity of absorbance of the final reaction is proportional to the quantity of brdu incorporated into cells. This assay is widely used in research to assess cell proliferation in vitro and in vivo, and can be modified to generate more targeted results.

Typology: Essays (university)

2018/2019

Uploaded on 03/23/2019

Jamse1028
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BrdU Cell Proliferation Assay
Halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine
(BrdU) can be used to label nascent DNA in viable cells. During the process
of DNA replication, BrdU can replace thymidine and incorporate into the
synthesized DNA of actively dividing cells. Since this BrdU reagent does not
cross react with endogenous DNA, cell proliferation thus can be reflected in
the extent of BrdU incorporation by determining the intensity of absorbance of
the final reaction. The BrdU cell proliferation assay is a versatile and
convenient method to quantify cell proliferation.
In the BrudU cell proliferation assay, cells are cultured with labeling medium
that contains BrdU and BrdU is incorporated into de novo-synthesized DNA
as a substitute for thymidine, which could label proliferating and daughter cells
until it is diluted out through multiple rounds of cell division. Cells proliferation
can be labeled by this method and BrdU can be detected by specific
monoclonal antibodies. There are several kinds of antibodies that can be used
to recognize the bound detection antibody. To achieve a better result, HRP
substrate TMB is added to develop color and the magnitude of the
absorbance for the developed color is proportional to the quantity of BrdU
incorporated into cells. Cell proliferation can be directly indicated by the
quantity of BrdU that is incorporated into cells. The BrdU cell proliferation
assay has been widely applied in the study of cell proliferation and this
method can also be used to assess cell proliferation in vivo.
The BrdU cell proliferation assay is a rapid, simple and high sensitive method
to assess cell proliferation. With the high resolution data of cell proliferation
acquired from this assay, a further understanding of the mechanisms of cell
proliferation can be achieved and many molecular mediators that could regulate
cell proliferation can be identified including hormones, signaling components
and transcriptional factors. Custom service is also available. This assay can be
modified to generate a more targeted result according to the purpose of the
research.

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BrdU Cell Proliferation Assay

Halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine (BrdU) can be used to label nascent DNA in viable cells. During the process of DNA replication, BrdU can replace thymidine and incorporate into the synthesized DNA of actively dividing cells. Since this BrdU reagent does not cross react with endogenous DNA, cell proliferation thus can be reflected in the extent of BrdU incorporation by determining the intensity of absorbance of the final reaction. The BrdU cell proliferation assay is a versatile and convenient method to quantify cell proliferation. In the BrudU cell proliferation assay, cells are cultured with labeling medium that contains BrdU and BrdU is incorporated into de novo-synthesized DNA as a substitute for thymidine, which could label proliferating and daughter cells until it is diluted out through multiple rounds of cell division. Cells proliferation can be labeled by this method and BrdU can be detected by specific monoclonal antibodies. There are several kinds of antibodies that can be used to recognize the bound detection antibody. To achieve a better result, HRP substrate TMB is added to develop color and the magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells. Cell proliferation can be directly indicated by the quantity of BrdU that is incorporated into cells. The BrdU cell proliferation assay has been widely applied in the study of cell proliferation and this method can also be used to assess cell proliferation in vivo. The BrdU cell proliferation assay is a rapid, simple and high sensitive method to assess cell proliferation. With the high resolution data of cell proliferation acquired from this assay, a further understanding of the mechanisms of cell proliferation can be achieved and many molecular mediators that could regulate cell proliferation can be identified including hormones, signaling components and transcriptional factors. Custom service is also available. This assay can be modified to generate a more targeted result according to the purpose of the research.