Biology 211 Name : Sam Tian Date: 10/26/21
Gene Expression ICA
Transcription comparison
Compare and contrast transcription in prokaryotes and eukaryotes.
Prokaryotes Eukaryotes
Polymerases - 1 RNA polymerase - 3 polymerases
- RNA pol II for
transcription
Promoter - -10(TATA) and -35
(TTAGACA)
sequence
- Core promoter
- Proximal promoter
- Distal promoter
-
Transcription factor(s) - Sigma factor - GTF (general
transcription
factor) binds to
core
- GSTFs (gene
specific
transcription
factor) binds to
distal
mRNA processing - none 1.) 3’ Poly A tail added
2.) intron splicing
3.) alternative (diff.
exon) splicing
Transcription
termination
- RNA forms a
hairpin (binding
back on itself)
- Snapping
termination
sequence off
(sequence of U’s,
A-U hydrogen
bonds broken)
- Poly-A termination
sequence, then
CPSF (cleavage
and
polyadenylation
specificity factor)
and CstF (cleavage
stimulation factor)
cleaves the mRNA
off
Spatial-temporal
regulation
- Yes, transcription
and translation can
occur at the same
time (since it all
takes place in the
- No (transcription
occurs in nucleus,
translation occurs
in the cytoplasm)
