TERM 1
BIOL 308 - Lecture 5 What is the rate of
renaturation?
DEFINITION 1
-measure of complexity of DNA/genome
TERM 2
What is the speed at which a single strand is
able to find a complementary strand and base
pair with it?
DEFINITION 2
Reassociation kinetics
TERM 3
What is CoT used to measure? What is Co?
What is t?
DEFINITION 3
CoT measures renaturation.Co - intial concentrationt =
reaction time
TERM 4
What are the three main classes of DNA in
EUKARYOTIC genomes?
DEFINITION 4
-unique --> one to few copies, slow renaturation, mostly
protein coding sequences-moderately repetitive --> middle
renaturation, code for some protein families as well as tRNA
and rRNA, some are transposable-highly repetitive --> lots of
them, fast renaturation, unknown role and mostly in the
telomere-centromere regions
TERM 5
E.Coli genome(2) vs. Calf genome (2)
DEFINITION 5
E.Coli:-largely unique-once sequences are found, fast
reassociationCalf:-fast reassociation-some unique
sequences: start slow but then get faster as it moves along
