Download DNA Replication: Enzymes, Processes, and Role of DNA Polymerase and Topoisomerase II and more Quizzes Molecular biology in PDF only on Docsity!
What is the enzyme responsible for synthesis
of new strands?
DNA polymerase TERM 2
How does polymerization happen? (generally)
DEFINITION 2 Nucleotide base pairs with template strand and 3' OH of the primer binds to the alpha phosphate TERM 3
DNA pol III: What happens at the alpha site?
"E" site? Theta site? What does the B subunit
do?
DEFINITION 3 5'-3' polymerization 3'-5' exonuclease (hydrolyze + expel incorrect base) Unknown At 3' end of growing strand, increases processivity TERM 4
Remaining subunits form the gamma
complex. What does it do?
DEFINITION 4 -involved in opening and closing of clamp -loading beta subunit onto DNA template -unloading beta subunit TERM 5
The gamma complex includes two subunits of
____. Which helps with what?
DEFINITION 5 t - helps dimerization of two core polymerases
What is the role of RNase H?
Removes RNA primers --> 3-5' exonuclease activity TERM 7
What are the the two exonuclease activities of
DNA pol I?
DEFINITION 7 -5-3' removal of RNA primers and replace with DNA primers- 3-5' proofreading TERM 8
What is the role of DNA
ligase?
DEFINITION 8 -links fragments on the lagging strand-makes phosphodiester bond between 3' and 5' ends TERM 9
Topoisomerase II: What does it require? List
the two major roles.
DEFINITION 9 -requires ATP Introduces negative supercoiul around OriC (necessary for intiation of rep.) Helicase creates positive supercoils --> TOPOII makes them negative TERM 10
Whats wrong with positive super coils?
DEFINITION 10 Not energetically favorable
