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Definitions and functions of key enzymes and processes involved in dna replication, including dna polymerase, rnase h, dna ligase, and topoisomerase ii. Learn about the roles of these enzymes in synthesizing new strands, polymerization, exonuclease activities, and the formation of okazaki fragments.
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DNA polymerase TERM 2
DEFINITION 2 Nucleotide base pairs with template strand and 3' OH of the primer binds to the alpha phosphate TERM 3
DEFINITION 3 5'-3' polymerization 3'-5' exonuclease (hydrolyze + expel incorrect base) Unknown At 3' end of growing strand, increases processivity TERM 4
DEFINITION 4 -involved in opening and closing of clamp -loading beta subunit onto DNA template -unloading beta subunit TERM 5
DEFINITION 5 t - helps dimerization of two core polymerases
Removes RNA primers --> 3-5' exonuclease activity TERM 7
DEFINITION 7 -5-3' removal of RNA primers and replace with DNA primers- 3-5' proofreading TERM 8
DEFINITION 8 -links fragments on the lagging strand-makes phosphodiester bond between 3' and 5' ends TERM 9
DEFINITION 9 -requires ATP Introduces negative supercoiul around OriC (necessary for intiation of rep.) Helicase creates positive supercoils --> TOPOII makes them negative TERM 10
DEFINITION 10 Not energetically favorable