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DNA Replication: Enzymes, Processes, and Role of DNA Polymerase and Topoisomerase II, Quizzes of Molecular biology

Definitions and functions of key enzymes and processes involved in dna replication, including dna polymerase, rnase h, dna ligase, and topoisomerase ii. Learn about the roles of these enzymes in synthesizing new strands, polymerization, exonuclease activities, and the formation of okazaki fragments.

Typology: Quizzes

2010/2011

Uploaded on 10/12/2011

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TERM 1
What is the enzyme responsible for synthesis
of new strands?
DEFINITION 1
DNA polymerase
TERM 2
How does polymerization happen? (generally)
DEFINITION 2
Nucleotide base pairs with template strand and 3' OH of the
primer binds to the alpha phosphate
TERM 3
DNA pol III: What happens at the alpha site?
"E" site? Theta site? What does the B subunit
do?
DEFINITION 3
5'-3' polymerization
3'-5' exonuclease (hydrolyze + expel incorrect base)
Unknown
At 3' end of growing strand, increases processivity
TERM 4
Remaining subunits form the gamma
complex. What does it do?
DEFINITION 4
-involved in opening and closing of clamp -loading beta
subunit onto DNA template -unloading beta subunit
TERM 5
The gamma complex includes two subunits of
____. Which helps with what?
DEFINITION 5
t - helps dimerization of two core polymerases
pf3
pf4

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What is the enzyme responsible for synthesis

of new strands?

DNA polymerase TERM 2

How does polymerization happen? (generally)

DEFINITION 2 Nucleotide base pairs with template strand and 3' OH of the primer binds to the alpha phosphate TERM 3

DNA pol III: What happens at the alpha site?

"E" site? Theta site? What does the B subunit

do?

DEFINITION 3 5'-3' polymerization 3'-5' exonuclease (hydrolyze + expel incorrect base) Unknown At 3' end of growing strand, increases processivity TERM 4

Remaining subunits form the gamma

complex. What does it do?

DEFINITION 4 -involved in opening and closing of clamp -loading beta subunit onto DNA template -unloading beta subunit TERM 5

The gamma complex includes two subunits of

____. Which helps with what?

DEFINITION 5 t - helps dimerization of two core polymerases

What is the role of RNase H?

Removes RNA primers --> 3-5' exonuclease activity TERM 7

What are the the two exonuclease activities of

DNA pol I?

DEFINITION 7 -5-3' removal of RNA primers and replace with DNA primers- 3-5' proofreading TERM 8

What is the role of DNA

ligase?

DEFINITION 8 -links fragments on the lagging strand-makes phosphodiester bond between 3' and 5' ends TERM 9

Topoisomerase II: What does it require? List

the two major roles.

DEFINITION 9 -requires ATP Introduces negative supercoiul around OriC (necessary for intiation of rep.) Helicase creates positive supercoils --> TOPOII makes them negative TERM 10

Whats wrong with positive super coils?

DEFINITION 10 Not energetically favorable