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Biochemistry Chapter 17 | PHAR - Pharmacology, Quizzes of Pharmacology

Class: PHAR - Pharmacology; Subject: Pharmacology; University: Lake Erie College of Osteopathic Medicine; Term: Forever 1989;

Typology: Quizzes

2013/2014

Uploaded on 12/10/2014

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TERM 1
Plasmid
DEFINITION 1
A vector that carried foreign DNA
TERM 2
3 components of a plasmid
DEFINITION 2
Restriction enzymesorigin of replicationOne or more
restriction sites
TERM 3
Vector
DEFINITION 3
DNA molecule that can replicated autonomously in the host
cell
TERM 4
Chimeric Plasmid
DEFINITION 4
Foreign DNA and plasmid form together
TERM 5
A plasmid can have a foreign DNA particle
inserted up to ____ kb in size
DEFINITION 5
up to 2.0kb in size
pf3
pf4
pf5

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Plasmid

A vector that carried foreign DNA

TERM 2

3 components of a plasmid

DEFINITION 2

Restriction enzymesorigin of replicationOne or more

restriction sites

TERM 3

Vector

DEFINITION 3

DNA molecule that can replicated autonomously in the host

cell

TERM 4

Chimeric Plasmid

DEFINITION 4

Foreign DNA and plasmid form together

TERM 5

A plasmid can have a foreign DNA particle

inserted up to ____ kb in size

DEFINITION 5

up to 2.0kb in size

What do restriction enzymes look

for?

Palindromes: Specific double stranded sequences

TERM 7

What are 2 specific examples of restriction

enzymes?

DEFINITION 7

ECOr1SMA

TERM 8

Restriction enzymes are _____ and cleave

_____ backbones at or near the recognition

site

DEFINITION 8

Restriction enzymes are ENDOnucleases and cleave

phosphodiester backbones at or near the recognition site

TERM 9

What is a DNA library?

DEFINITION 9

Collection of restriction fragments clones within suitable host

cells

TERM 10

What is a collection of hybrid vectors with

cDNA inserts?

DEFINITION 10

a cDNA library

What is the role of risc during RNAi?

risc links the complex to the mRNA for silencing via base

pairing

TERM 17

What is the role of dicer during RNAi?

DEFINITION 17

Dicer cleaves dsRNA into small fragments that can be loaded

into the RISC complex

TERM 18

What is gene therapy?

DEFINITION 18

isolating normal genes and inserting them into diseased cells

for expression of normal cell traits

TERM 19

what is gene ablation and how is it achieved?

DEFINITION 19

Gene ablation is removal of a set of genesit is accomplished

by siRNA which inhibits translation

TERM 20

T/R RNAi silences genes while SiRNA inhibits

translation

DEFINITION 20

TrueSiRNA can also cause degradation of mRNA

genetic markers

polymorphisms that occur and are linked to genes involved

in an inherited disease

TERM 22

proteomics

DEFINITION 22

study of proteins expressed by a cell

TERM 23

What are 4 uses for PCR

DEFINITION 23

Sensitive detection (contaiminents in air, food, water,

tissue)Medical diagnostic testsForensic detectionGenotyping