BIOCHEMISTRY PART
I1
Exam
#1
and
#2
Chapter
38
1.
Martin Rodbell frrst described the
G
proteins in 1971 (pg261)
2.
In
the resting cell the
G
protein norrnally lies adjacent to the receptor
in
an inactive off
state
@g263)
3.
An
example of a monomeric
G
protein is Ras (pg265)
4.
Gp
stimulates phospholipase c (pg263)
Chapter
39
1.
Target cells have
high
affinity receptors that convey specificity
2. Insulin receptor is tetrameric
3.
Receptors with endogenous tyrosine kinase activity are capable of autophosphorylation.
4. Peptide hormones
hulin
binds to tetmmeric receptors
5.
Insdin signal transduction mechanism
6.
Steroid hormones bind to intracellular bi-firnctional receptor proteins
Chapter
40
1.
DNA polynucleotide chain has specific directionality, growth occurs at 3'end
2. Know the different chargaffs rules
3. Know structure of Eukaryotic chromosomes
4.
Prokaryotic DNA is organized into operons
5.
Know that human mitochondrial DNA
is
circular
Chapter
41
1.
Most significant amount of experimental support is found in the semiconservative
model
2.
In
DNA replication, replication proceeds bidirectionally and semiconservatively
3.
Know the requirements for DNA replication
Chapter
42
1. Know the steps
in
replication
2. Know the role of the primase RNA polymerase
3.
The lagging strand grows away fiom the replication fork
4.
As
we age we loose genetic material
5. Telomere replication
6.
Each origin has two replication forks
7. Inhibitors of reverse transcriptase are acyclovir
and
AZT
.
8.
Telomerase prevents aging and loss of genetic material
Chapter
43
1.
Single amino acid change from
glutamate
to valine by single base change
A
-
U
is
responsible for sickle cell anemia
2.
Alpha thalassernia is an example of nonsense mutation
3. Base excision repair (pg305)
Chapter
44
1. tRNA
has
a dual role of polarity
2. rRNA has both structural and enzymatic roles
Chapter
49
