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MICRO LAB EXAM 1 REVISION
- i. Identify a likely genus and species of this organism. ii. What Gram stain type are these bacteria?
i. Staphylococcus epidermidis Staphylococci - clusters of circular bacteria Staphylococcus Epidermidis – ii. Gram positive (Gram +(purple) cocci
- i. What is the bacterial arrangement? ii. What is the shape of the identify organism?
i. Cluster (Staphylococci - clusters of circular bacteria) ii. cocci
- What is the proper way to carry a microscope? Both hands, one hand holding the arm & the other under the base.
- Define Ubiquitous present or existing everywhere
- What focusing with low power objectives (4X or 10X)? Course focus Or What is the function of the course adjustment knob? focusing with low power objectives (4X or 10X) NOTE : What is the function of the fine adjustment knob on a microscope? Focuses the high power and oil immersion objectives
- Name the labeled structure on the microscope, and its function. Ocular lens
- Name the dye used to color the spores
Malachite green In the Schaeffer-Fulton endospore stain, what is the primary stain? Malachite green NOTE: Stain type(s), stain, specimens spore stain (+)
**_- malachite green
- sps (+) | Bacillus subtilis (#2), Bacillus cereus_**
- Protista
- List two acid fast genera: Mycobacterium and Nocardia 16.. i. Identify a likely genus and species of this organism. ii. What Gram stain type are these bacteria? iii. What is the bacterial arrangement?
i. Serratia marcescens – ii. Gram negative (Gram – ) (pink) bacilli
- i. Identify the likely genus and species of this organism. ii. What Gram stain type are these bacteria?
i. Escherichia coli E. Coli - Gram - (pink) bacilli ii. Gram-negative
- Identify the shape of these bacterial cells? Rod-shaped/ coccobacillus ii. What is the arrangement of these bacterial cells? Singles Which microorganism is a common, normal inhabitant of the human intestine? Escherichia coli is part of its normal flora.
- TTC agar. Which organism is the organism motile--left, right, or both?
Left Broth
- Slant
- The melting temperature of agar is? At 100^0 C
- The solidification temperature of agar is? At 40^0 C
- What is the purpose of immersion oil for viewing specimens? Reduce refraction or bending of light and increase resolution Prevent refraction The immersion oil decreases refraction of light Note: 100X Objective is always used with immersion oil
- What is the cause of Brownian movement? Brownian movement is caused by the constant, random motion of fluid molecules or atoms impacting and pushing larger particles Brownian movement Why is petroleum jelly used to make this slide? reduces evaporation of the suspended drop of fluid.
- Name two methods for determining motility in bacteria? wet mount; hanging drop preparation; motility agar; flagellar staining Wet mount preparation/ Hanging drop- Agar Stain You look through a monocular microscope and observe a wet mount preparation of bacterial cells. Sample Questions: Are these cells moving? Explain. Yes -- all cells in a wet mount are moving by Brownian motion
- Primary stain that is purple
- Which organism is + on this methylene blue lipid agar plate?
left Methylane blue
- i. What is the genus and species name of this organism? ii. To what kingdom does this organism belong?
Trichinella spiralis Genus: Trichinella Kingdom Animalia Shape : spiral-shaped, parasitic worm
- i. what mechanism do these organisms reproduce? ii. Name one disease caused by these organisms Disease caused : trichinosis
- One
- What does CFU stand for? OR The letters CFU stand for Colony Forming Units
- What is the genus and species name of this organism?
Aspergillus niger Aspergillus - bluish-green with sulfur-yellow areas on the surface
- i. To what kingdom do these organisms belong? Kingdom fungi ii. To what Domain do these organisms belong? Domain: Eukaryote
- i. Identify the likely genus of the organism below ii. To what kingdom do these organisms belong?
Rhizopus Rhizopus nigricans Kingdom: Fungi Genus: Rhizopus
- Spores (Rhizopus - a Zygomycetes; spores with dark pigment)
- if an object is centered and in sharp focus with one objective, it will be centered and in focus when another object is rotated to its viewing position is known as Paracentric
- Resolution – increases with increase in magnification C. Resolution NOTE: Relationship of magnification with: i. Field of view – inversely proportional (decreases with increase in magnification ii. Working distance – decreases with increase in magnification iii. Depth of field – decreases with increase in magnification iv. Resolution – increases with increase in magnification
