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Molecular Biology Exam: DNA Replication, Transcription, and Immunology Questions, Exams of Biology

A series of questions and answers related to molecular biology, specifically focusing on dna replication, transcription, and translation. It covers key concepts such as the role of rna polymerase, the function of ribosomes, the process of dna replication being semiconservative, and the importance of the tata box in eukaryotic promoters. Additionally, it touches on immunology, including autoimmune diseases and vaccine mechanisms. The questions are designed to test understanding of these fundamental processes and their components, making it a useful review tool for students studying molecular biology and genetics. The document also includes questions about the immune system, such as the role of antibodies and the differences between innate and adaptive immunity. It serves as a comprehensive review of essential topics in biology, suitable for exam preparation or reinforcing core concepts.

Typology: Exams

2024/2025

Available from 06/06/2025

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Bio 5 L112 5 Exam 5 3 5 Practice, 5 Exam 5 4 5
review, 5 L112 5 Final 5 Exam 5 Review, 5
BIOL-L 5 112 5 Final 5 Exam 5 with 5 verified 5
answers
A 5 polyribosome 5 is 5 - 5 Correct 5 answer 5 a 5 mRNA 5 molecule 5 that 5 is 5 bound 5 to 5
many 5 ribosomes 5 in 5 the 5 act 5 of 5 translation
The 5 place 5 where 5 RNA 5 polymerase 5 first 5 associates 5 with 5 DNA 5 so 5 that 5
transcription 5 can 5 begin 5 is 5 called 5 the 5 __________ 5 and 5 is 5 located 5 ____________ 5 of 5 the 5
transcribed 5 region 5 - 5 Correct 5 answer 5 promoter; 5 upstream
Which 5 of 5 the 5 following 5 add 5 individual 5 nucleotides 5 to 5 the 5 3' 5 end 5 of 5 an 5
existing 5 strand 5 to 5 build 5 a 5 new 5 DNA 5 strand 5 during 5 replication 5 - 5 Correct 5
answer 5 DNA 5 polymerase
Picture 5 of 5 a 5 big 5 balloon 5 thing 5 wrapped 5 around 5 a 5 section 5 of 5 DNA, 5 what's 5
the 5 big 5 balloon 5 thing? 5 - 5 Correct 5 answer 5 a 5 RNA 5 polymerase
Translation 5 ends 5 when 5 a 5 stop 5 codon 5 is 5 at 5 the 5 _____ 5 site, 5 allowing 5 a 5 ________ 5 to 5
bind 5 there 5 - 5 Correct 5 answer 5 A; 5 release 5 factor
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Download Molecular Biology Exam: DNA Replication, Transcription, and Immunology Questions and more Exams Biology in PDF only on Docsity!

Bio 5 L112 5 Exam 5 3 5 Practice, 5 Exam 5 4 5

review, 5 L112 5 Final 5 Exam 5 Review, 5

BIOL-L 5 112 5 Final 5 Exam 5 with 5 verified 5

answers

A 5 polyribosome 5 is 5 - 5 Correct 5 answer 5 ✔a 5 mRNA 5 molecule 5 that 5 is 5 bound 5 to 5 many 5 ribosomes 5 in 5 the 5 act 5 of 5 translation The 5 place 5 where 5 RNA 5 polymerase 5 first 5 associates 5 with 5 DNA 5 so 5 that 5 transcription 5 can 5 begin 5 is 5 called 5 the 5 __________ 5 and 5 is 5 located 5 ____________ 5 of 5 the 5 transcribed 5 region 5 - 5 Correct 5 answer 5 ✔promoter; 5 upstream Which 5 of 5 the 5 following 5 add 5 individual 5 nucleotides 5 to 5 the 5 3' 5 end 5 of 5 an 5 existing 5 strand 5 to 5 build 5 a 5 new 5 DNA 5 strand 5 during 5 replication 5 - 5 Correct 5 answer 5 ✔DNA 5 polymerase Picture 5 of 5 a 5 big 5 balloon 5 thing 5 wrapped 5 around 5 a 5 section 5 of 5 DNA, 5 what's 5 the 5 big 5 balloon 5 thing? 5 - 5 Correct 5 answer 5 ✔a 5 RNA 5 polymerase Translation 5 ends 5 when 5 a 5 stop 5 codon 5 is 5 at 5 the 5 _____ 5 site, 5 allowing 5 a 5 ________ 5 to 5 bind 5 there 5 - 5 Correct 5 answer 5 ✔A; 5 release 5 factor

DNA 5 replication 5 must 5 start 5 at 5 a 5 replication 5 origin. 5 In 5 eukaryotes 5 that 5 DNA 5 molecule 5 that 5 makes 5 up 5 a 5 chromosome 5 is 5 typically 5 __________ 5 and 5 usually 5 has 5 _________ 5 replication 5 origin. 5 - 5 Correct 5 answer 5 ✔linear, 5 more 5 than 5 one The 5 start 5 codon 5 translation 5 direction 5 incorporation 5 of 5 which 5 amino 5 acid? 5 - 5 Correct 5 answer 5 ✔methionine Which 5 of 5 the 5 following 5 can 5 be 5 safely 5 used 5 in 5 a 5 vaccine? 5 - 5 Correct 5 answer 5 ✔a 5 killed 5 pathogen 5 AND 5 a 5 weakened 5 live 5 pathogen The 5 central 5 dogma 5 describes 5 the 5 flow 5 of 5 information 5 in 5 eukaryotic 5 gene 5 expression 5 as 5 - 5 Correct 5 answer 5 ✔DNA 5 ----> 5 RNA 5 ----> 5 protein Reiji 5 Okazaki 5 discovered 5 what 5 are 5 now 5 called 5 Okazaki 5 fragment 5 which 5 are 5 produced 5 during 5 DNA 5 replication. 5 These 5 are 5 - 5 Correct 5 answer 5 ✔short 5 length 5 of 5 new 5 DNA 5 on 5 the 5 lagging 5 strand DNA 5 replication 5 is 5 said 5 to 5 be 5 semiconservative 5 because 5 - 5 Correct 5 answer 5 ✔each 5 new 5 DNA 5 molecule 5 is 5 composed 5 of 5 one 5 old 5 strand 5 and 5 one 5 new 5 strand The 5 TATA 5 box 5 is 5 a 5 key 5 element 5 of 5 the 5 _______ 5 of 5 most 5 eukaryotic 5 protein- coding 5 genes 5 - 5 Correct 5 answer 5 ✔promoter

How 5 many 5 different 5 kinds 5 of 5 RNA 5 codons 5 are 5 there 5 in 5 eukaryotes? 5 - 5 Correct 5 answer 5 ✔ 64 Telomere 5 characteristics 5 include 5 the 5 following 5 EXCEPT 5 - 5 Correct 5 answer 5 ✔increase 5 with 5 senescence 5 (aging) The 5 process 5 of 5 removing 5 introns 5 from 5 pre-mRNA 5 and 5 splicing 5 the 5 remaining 5 exons 5 together 5 occurs 5 in 5 a 5 complex 5 called 5 the 5 ____________ 5 - 5 Correct 5 answer 5 ✔signal 5 sequence Mother's 5 milk 5 is 5 considered 5 highly 5 beneficial 5 to 5 infant 5 immunity 5 because 5 if 5 contains 5 an 5 antibody. 5 This 5 antibody 5 is 5 called 5 an 5 _________, 5 is 5 found 5 in 5 mother's 5 milk 5 and 5 in 5 the 5 ____________ 5 - 5 Correct 5 answer 5 ✔IgA, 5 saliva Coding 5 strand: 5 5' 5 GCATTACCA 5 3'. 5 which 5 of 5 the 5 following 5 sequence 5 is 5 the 5 correct 5 transcript 5 from 5 that 5 region? 5 - 5 Correct 5 answer 5 ✔5' 5 GCAUUACCA 5 3' All 5 of 5 the 5 following 5 are 5 innate 5 defense 5 mechanisms 5 EXCEPT 5 - 5 Correct 5 answer 5 ✔antibodies Prior 5 to 5 the 5 1940s, 5 many 5 biologists 5 thought 5 that_____________, 5 being 5 made 5 up 5 of 5 20 5 types 5 of 5 ________, 5 was 5 the 5 best 5 candidate 5 fro 5 genetic 5 material 5 - 5 Correct 5 answer 5 ✔protein; 5 amino 5 acids As 5 a 5 student 5 in 5 this 5 course 5 you 5 learned 5 that 5 this 5 particular 5 antibody 5 is 5 considered 5 'bad' 5 when 5 present 5 at 5 elevated 5 levels. 5 However 5 there 5 is 5 an 5

advantage 5 to 5 its 5 presence. 5 What 5 can 5 this 5 antibody 5 do 5 that 5 other 5 classes 5 of 5 antibodies 5 are 5 unable 5 to 5 do? 5 - 5 Correct 5 answer 5 ✔it 5 kills 5 parasitc 5 worms Which 5 of 5 the 5 following 5 carries 5 amino 5 acids 5 to 5 ribosomes, 5 where 5 amino 5 acids 5 are 5 linked 5 into 5 the 5 primary 5 structure 5 of 5 a 5 polypeptide? 5 - 5 Correct 5 answer 5 ✔tRNA Replication 5 of 5 DNA 5 in 5 eukaryotes 5 - 5 Correct 5 answer 5 ✔is 5 semi-conservative A 5 class 5 of 5 antibodies 5 is 5 able 5 to 5 cross 5 the 5 placenta 5 from 5 mother 5 to 5 fetus. 5 This 5 confers 5 immunity 5 to 5 the 5 newborn 5 before 5 it 5 is 5 able 5 to 5 develop 5 its 5 own 5 immunity. 5 Name 5 the 5 antibody 5 class: 5 - 5 Correct 5 answer 5 ✔IgG Each 5 DNA 5 nucleotide 5 is 5 made 5 up 5 of 5 - 5 Correct 5 answer 5 ✔a 5 five-carbon 5 sugar, 5 a 5 phosphate 5 group, 5 and 5 one 5 of 5 four 5 nitrogenous 5 bases In 5 what 5 stage 5 of 5 translation 5 does 5 the 5 peptide 5 bond 5 join 5 two 5 amino 5 acids? 5 - 5 Correct 5 answer 5 ✔elongation The 5 portions 5 of 5 a 5 gene 5 that 5 are 5 transcribed 5 but 5 not 5 translated 5 are 5 called 5 - 5 Correct 5 answer 5 ✔introns Frameshift 5 mutations 5 typically 5 involve 5 - 5 Correct 5 answer 5 ✔addition 5 or 5 deletion 5 of 5 one 5 or 5 several 5 base 5 pairs

The 5 human 5 immunodeficiency 5 virus 5 (HIV) 5 is 5 particularly 5 damaging 5 to 5 humans 5 because 5 it 5 - 5 Correct 5 answer 5 ✔mutates 5 frequently 5 and 5 kills 5 helper 5 T 5 cells Which 5 of 5 the 5 following 5 acts 5 as 5 'filters' 5 in 5 the 5 lymphatic 5 system? 5 - 5 Correct 5 answer 5 ✔lymph 5 nodes Which 5 of 5 the 5 following 5 is 5 correct 5 regarding 5 the 5 contribution(s) 5 made 5 by 5 James 5 Watson 5 and 5 Francis 5 Crick? 5 - 5 Correct 5 answer 5 ✔They 5 established 5 the 5 double-stranded 5 nature 5 of 5 DNA, they 5 established 5 the 5 principle 5 of 5 base 5 pairing 5 based 5 onf 5 R. 5 Franklin's 5 data, They 5 explained 5 how 5 DNA's 5 structure 5 permitted 5 it 5 to 5 be 5 replicated, AND they 5 proposed 5 the 5 concept 5 of 5 the 5 double 5 helic The 5 two 5 strands 5 of 5 a 5 DNA 5 double 5 helix 5 are 5 said 5 to 5 be 5 antiparallel. 5 This 5 means 5 that 5 - 5 Correct 5 answer 5 ✔since 5 the 5 double 5 helix 5 twists, 5 it 5 is 5 not 5 perfectly 5 parallel Suppose 5 that 5 the 5 first 5 part 5 of 5 a 5 new 5 polypeptide 5 chain 5 being 5 produced 5 in 5 a 5 eukaryotic 5 cell 5 does 5 not 5 have 5 a 5 signal 5 peptide. 5 Where 5 would 5 you 5 expect 5 translation 5 to 5 take 5 place? 5 - 5 Correct 5 answer 5 ✔rough 5 ER 5 only Which 5 of 5 the 5 following 5 nitrogenous 5 bases 5 in 5 NOT 5 normally 5 found 5 in 5 DNA? 5

  • 5 Correct 5 answer 5 ✔uracil

A 5 ribozyme 5 is 5 - 5 Correct 5 answer 5 ✔an 5 RNA 5 capable 5 of 5 catalyzing 5 reactions 5 similar 5 to 5 the 5 action 5 of 5 protein 5 enzymes Coding 5 strand: 5 5' 5 GCATTACCA 5 3' Consider 5 the 5 first 5 A 5 nucleotide 5 in 5 the 5 coding 5 strand 5 is 5 mutated 5 to 5 a 5 C. 5 This 5 would 5 result 5 in 5 a 5 ____________ 5 mutation 5 - 5 Correct 5 answer 5 ✔silent 5 mutation DNA 5 and 5 RNA 5 are 5 alike 5 in 5 _______ a. 5 pentose 5 sugar b. 5 all 5 bases 5 used 5 to 5 assemble 5 genetic 5 code c. 5 having 5 same 5 role 5 in 5 transcription d. 5 their 5 3d 5 structure e. 5 none 5 of 5 the 5 above 5 - 5 Correct 5 answer 5 ✔none 5 of 5 these 5 are 5 correct The 5 region 5 in 5 a 5 tRNA 5 that 5 bonds 5 with 5 mRNA 5 during 5 translation 5 is 5 the 5 _________ 5 - 5 Correct 5 answer 5 ✔anticodon The 5 DNA 5 in 5 the 5 nucleus 5 of 5 a 5 typical 5 human 5 cell 5 nucleus 5 would 5 be 5 about 5 _________ 5 long 5 if 5 fully 5 stretched 5 out. 5 - 5 Correct 5 answer 5 ✔ 2 5 m During 5 the 5 elongation 5 stage 5 of 5 transcription 5 - 5 Correct 5 answer 5 ✔RNA 5 nucleotides 5 are 5 added 5 to 5 the 5 transcript, 5 the 5 transcript 5 grows 5 in 5 the 5 5' 5 to 5 3' 5 direction, 5 RNA 5 polymerase 5 unwinds 5 the 5 DNA, 5 AND 5 the 5 double 5 helix 5 reforms 5 behind 5 RNA 5 polymerase

Start 5 codon 5 - 5 Correct 5 answer 5 ✔AUG 5 (methionine) Stop 5 Codons 5 - 5 Correct 5 answer 5 ✔UAG, 5 UAA, 5 UGA codon 5 - 5 Correct 5 answer 5 ✔The 5 basic 5 unit 5 of 5 the 5 unit 5 genetic 5 code. 5 A 5 three 5 nucleotide 5 sequence 5 sequence 5 of 5 DNA 5 or 5 mRNA 5 that 5 specifies 5 a 5 particular 5 amino 5 acid anticodon 5 - 5 Correct 5 answer 5 ✔a 5 nucleotide 5 triplet 5 at 5 one 5 end 5 of 5 the 5 tRNA 5 that 5 recognizes 5 a 5 particular 5 complimentary 5 codon 5 on 5 a 5 mRNA 5 molecule tRNA 5 - 5 Correct 5 answer 5 ✔an 5 RNA 5 molecule 5 that 5 functions 5 as 5 an 5 interpreter 5 between 5 nucleic 5 acid 5 and 5 protein 5 language 5 by 5 picking 5 up 5 a 5 specific 5 amino 5 acid 5 and 5 recognizing 5 the 5 appropriate 5 codon 5 in 5 the 5 mRNA rRNA 5 - 5 Correct 5 answer 5 ✔the 5 most 5 abundant 5 type 5 of 5 RNA 5 - 5 found 5 in 5 ribosome Amino 5 Acid 5 - 5 Correct 5 answer 5 ✔monomers 5 of 5 peptides aminoacyl 5 - 5 tRNA 5 synthetase 5 - 5 Correct 5 answer 5 ✔a 5 family 5 of 5 enzymes 5 that 5 function 5 to 5 correctly 5 match 5 tRNAs 5 and 5 amino 5 acids

tRNA 5 binding 5 site 5 A 5 - 5 Correct 5 answer 5 ✔site 5 in 5 the 5 ribosome 5 that 5 holds 5 tRNA 5 carrying 5 the 5 next 5 amino 5 acid, 5 tRNA 5 starts 5 here 5 & 5 codon 5 is 5 checked 5 with 5 anticodon, 5 if 5 correct, 5 moves 5 on 5 the 5 P 5 site tRNA 5 binding 5 site 5 P 5 - 5 Correct 5 answer 5 ✔site 5 in 5 the 5 ribosome 5 that 5 holds 5 the 5 tRNA 5 & 5 attaches 5 it 5 to 5 the 5 growing 5 polypeptide 5 chain, 5 tRNA 5 goes 5 to 5 E 5 site tRNA 5 binding 5 site 5 E 5 - 5 Correct 5 answer 5 ✔site 5 in 5 the 5 ribosome 5 that 5 holds 5 the 5 tRNA 5 to 5 be 5 discharged, 5 tRNA 5 goes 5 back 5 to 5 cytoplasm 5 to 5 pick 5 up 5 another 5 amino 5 acid release 5 factor 5 - 5 Correct 5 answer 5 ✔a 5 protein 5 shaped 5 like 5 tRNA 5 that 5 hydrolyzes 5 the 5 bond 5 between 5 the 5 tRNA 5 in 5 the 5 P 5 site 5 and 5 the 5 last 5 amino 5 acid 5 of 5 the 5 polypeptide 5 chain ribozyme 5 - 5 Correct 5 answer 5 ✔an 5 RNA 5 molecule 5 that 5 functions 5 like 5 an 5 enzyme. 5 During 5 translation, 5 rRNA 5 catalyzes 5 the 5 peptide 5 bond 5 between 5 the 5 new 5 amino 5 acid 5 in 5 the 5 tRNA 5 binding 5 site 5 A 5 and 5 the 5 growing 5 peptide 5 chain 5 in 5 the 5 tRNA 5 binding 5 site 5 P Lymphocytes 5 - 5 Correct 5 answer 5 ✔T 5 & 5 B 5 cells, 5 both 5 start 5 in 5 bone 5 marrow T 5 cells 5 - 5 Correct 5 answer 5 ✔leave 5 bone 5 marrow 5 and 5 mature 5 in 5 the 5 thymus B 5 cells 5 - 5 Correct 5 answer 5 ✔start 5 and 5 mature 5 in 5 the 5 bone 5 marrow

The 5 cap 5 on 5 an 5 mRNA 5 is 5 the 5 site 5 where a. 5 ribosomes 5 attach 5 at 5 the 5 start 5 of 5 translation b. 5 the 5 start 5 codon 5 is 5 located c. 5 the 5 process 5 of 5 translation 5 ends d. 5 the 5 stop 5 codon 5 is 5 covered 5 until 5 needed e. 5 the 5 mRNA 5 is 5 protected 5 from 5 attack 5 by 5 RNA-digesting 5 enzymes 5 - 5 Correct 5 answer 5 ✔a. 5 ribosomes 5 attach 5 at 5 the 5 start 5 of 5 translation During 5 the 5 elongation 5 stage 5 of 5 transcription a. 5 RNA 5 nucleotides 5 are 5 added 5 to 5 the 5 transcript b. 5 the 5 transcript 5 grows 5 in 5 the 5 5' 5 to 5 3' 5 direction c. 5 RNA 5 polymerase 5 unwinds 5 the 5 DNA d. 5 the 5 double 5 helix 5 reforms 5 behind 5 RNA 5 polymerase e. 5 all 5 of 5 these 5 - 5 Correct 5 answer 5 ✔e. 5 all 5 of 5 these Many 5 errors 5 made 5 during 5 DNA 5 replication 5 are 5 corrected 5 during 5 DNA 5 replication 5 through 5 proofreading 5 by 5 ______. a. 5 DNA 5 polymerase b. 5 primase c. 5 telomerase d. 5 DNA 5 ligase

e. 5 helicase 5 - 5 Correct 5 answer 5 ✔a. 5 DNA 5 polymerase During 5 DNA 5 replication, 5 the 5 _____ 5 strand 5 is 5 assembled 5 in 5 the 5 _______ 5 direction 5 that 5 the 5 DNA 5 double 5 helix 5 unwinds 5 and 5 is 5 produced 5 by 5 _____ 5 replication. a. 5 leading; 5 opposite; 5 continuous b. 5 lagging; 5 same; 5 discontinuous c. 5 leading; 5 same; 5 discontinuous d. 5 lagging; 5 opposite; 5 continuous e. 5 leading; 5 same; 5 continuous 5 - 5 Correct 5 answer 5 ✔e. 5 leading; 5 same; 5 continuous Which 5 of 5 the 5 following 5 assembles 5 a 5 short 5 RNA 5 chain 5 as 5 the 5 first 5 nucleotides 5 in 5 a 5 new 5 DNA 5 strand? a. 5 topoisomerase b. 5 primase c. 5 DNA 5 polymerase d. 5 helicase e. 5 DNA 5 ligase 5 - 5 Correct 5 answer 5 ✔b. 5 primase A 5 DNA 5 double 5 helix 5 has 5 two 5 strands 5 that 5 are 5 held 5 to 5 each 5 other 5 by 5 _____. a. 5 hydrogen 5 bonds b. 5 ionic 5 bonds

C) 5 ligase

  • B) 5 DNA 5 polymerase 5 III
  • D) 5 DNA 5 polymerase 5 I
  • The 5 leading 5 and 5 the 5 lagging 5 strands 5 differ 5 in 5 that E) 5 primase 5 - 5 Correct 5 answer 5 ✔E) 5 primase
  • A) 5 the 5 leading 5 strand 5 is 5 synthesized 5 in 5 the 5 same 5 direction 5 as 5 the 5 movement
  • 5 direction. of 5 the 5 replication 5 fork, 5 and 5 the 5 lagging 5 strand 5 is 5 synthesized 5 in 5 the 5 opposite
  • B) 5 the 5 leading 5 strand 5 is 5 synthe3ized 5 by 5 adding 5 nucleotides 5 to 5 the 5 3' 5 end 5 of
  • the 5 growing 5 strand, 5 and 5 the 5 lagging 5 strand 5 is 5 synthesized 5 by 5 adding
  • nucleotides 5 to 5 the 5 5' 5 end.
  • C) 5 the 5 lagging 5 strand 5 is 5 synthesized 5 continuously, 5 whereas 5 the 5 leading
  • strand 5 is 5 synthesized 5 in 5 short 5 fragments 5 that 5 are 5 ultimately 5 stitched
  • together.
  • D) 5 the 5 leading 5 strand 5 is 5 synthesized 5 at 5 twice 5 the 5 rate 5 of 5 the 5 lagging 5 strand.
    • 5 Correct 5 answer 5 ✔A) 5 the 5 leading 5 strand 5 is 5 synthesized 5 in 5 the 5 same
  • direction 5 as 5 the 5 movement 5 of 5 the 5 replication 5 fork, 5 and 5 the 5 lagging 5 strand 5 is
  • Which 5 of 5 the 5 following 5 best 5 describes 5 the 5 addition 5 of 5 nucleotides 5 to 5 a synthesized 5 in 5 the 5 opposite 5 direction.
  • growing 5 DNA 5 chain?
  • 5 molecule 5 of 5 pyrophosphate. A) 5 A 5 nucleotide 5 triphosphate 5 is 5 added 5 to 5 the 5 5' 5 end 5 of 5 the 5 DNA, 5 releasing 5 a

B) 5 A 5 nucleotide 5 triphosphate 5 is 5 added 5 to 5 the 5 3' 5 end 5 of 5 the 5 DNA, 5 releasing 5 a 5 molecule 5 of 5 pyrophosphate. 5 C) 5 A 5 nucleotide 5 diphosphate 5 is 5 added 5 to 5 the 5 5' 5 end 5 of 5 the 5 DNA, 5 releasing 5 a 5 molecule 5 of 5 phosphate. 5 D) 5 A 5 nucleotide 5 diphosphate 5 is 5 added 5 to 5 the 5 3' 5 end 5 of 5 the 5 DNA, 5 releasing 5 a 5 molecule 5 of 5 phosphate. 5 E) 5 A 5 nucleotide 5 monophosphate 5 is 5 added 5 to 5 the 5 5' 5 end 5 of 5 the 5 DNA. 5 - 5 Correct 5 answer 5 ✔B) 5 A 5 nucleotide 5 triphosphate 5 is 5 added 5 to 5 the 5 3' 5 end 5 of 5 the 5 DNA, 5 releasing 5 a 5 molecule 5 of 5 pyrophosphate. A 5 new 5 DNA 5 strand 5 elongates 5 only 5 in 5 the 5 5' 5 to 5 3' 5 direction 5 because 5 A) 5 DNA 5 polymerase 5 begins 5 adding 5 nucleotides 5 at 5 the 5 5' 5 end 5 of 5 the 5 template. 5 B) 5 Okazaki 5 fragments 5 prevent 5 elongation 5 in 5 the 5 3' 5 to 5 5' 5 direction. 5 C) 5 the 5 polarity 5 of 5 the 5 DNA 5 molecule 5 prevents 5 addition 5 of 5 nucleotides 5 at 5 the 5 3' 5 end. 5 D) 5 replication 5 must 5 progress 5 toward 5 the 5 replication 5 fork. 5 E) 5 DNA 5 polymerase 5 can 5 only 5 add 5 nucleotides 5 to 5 the 5 free 5 3' 5 end. 5 - 5 Correct 5 answer 5 ✔E) 5 DNA 5 polymerase 5 can 5 only 5 add 5 nucleotides 5 to 5 the 5 free 5 3' 5 end. What 5 is 5 the 5 role 5 of 5 DNA 5 ligase 5 in 5 the 5 elongation 5 of 5 the 5 lagging 5 strand 5 during 5 DNA 5 replication? 5 A) 5 synthesize 5 RNA 5 nucleotides 5 to 5 make 5 a 5 primer 5 B) 5 catalyze 5 the 5 lengthening 5 of 5 telomeres 5

A. 5 every 5 amino 5 acid 5 is 5 coded 5 for 5 by 5 a 5 single 5 codon. 5 B. 5 there 5 are 5 more 5 amino 5 acids 5 than 5 there 5 are 5 codons. 5 C. 5 every 5 codon 5 codes 5 for 5 an 5 amino 5 acid. 5 D. 5 each 5 codon 5 in 5 a 5 gene 5 codes 5 for 5 no 5 more 5 than 5 one 5 single 5 amino 5 acid. 5 - 5 Correct 5 answer 5 ✔D. 5 each 5 codon 5 in 5 a 5 gene 5 codes 5 for 5 no 5 more 5 than 5 one 5 single 5 amino 5 acid. Which 5 of 5 the 5 following 5 represent 5 three 5 posttranscriptional 5 modifications 5 often 5 seen 5 in 5 the 5 maturation 5 of 5 mRNA 5 in 5 eukaryotes? 5 A. 5 3'-capping, 5 5'-poly(A) 5 tail 5 addition, 5 splicing. 5 B. 5 removal 5 of 5 exons, 5 insertion 5 of 5 introns, 5 capping. 5 C. 5 5'-capping, 5 3'-poly(A) 5 tail 5 addition, 5 splicing. 5 D. 5 5'-poly(A) 5 tail 5 addition, 5 insertion 5 of 5 introns, 5 capping. 5 - 5 Correct 5 answer 5 ✔C. 5 5'-capping, 5 3'-poly(A) 5 tail 5 addition, 5 splicing. RNA 5 polymerase 5 binds 5 to 5 the: A. 5 operator B. 5 repressor C. 5 inducer D. 5 promoter 5 - 5 Correct 5 answer 5 ✔D. 5 promoter

Protein-encoding 5 genes 5 in 5 eukaryotes 5 are 5 transcribed 5 by A. 5 RNA 5 polymerase 5 I B. 5 RNA 5 polymerase 5 II C. 5 RNA 5 polymerase 5 III D. 5 RNA 5 polymerase 5 IV 5 - 5 Correct 5 answer 5 ✔B. 5 RNA 5 polymerase 5 II In 5 general, 5 methylation 5 of 5 DNA 5 in 5 eukaryotes A. 5 induces 5 genetic 5 rearrangements B. 5 activates 5 gene 5 expression C. 5 promotes 5 cell 5 growth D. 5 reduces 5 levels 5 of 5 gene 5 expression 5 - 5 Correct 5 answer 5 ✔D. 5 reduces 5 levels 5 of 5 gene 5 expression A 5 restriction 5 enzyme A. 5 cuts 5 DNA 5 at 5 random B. 5 degrades 5 RNA C. 5 normally 5 cleaves 5 DNA 5 within 5 a 5 specific 5 sequence D. 5 none 5 of 5 the 5 above 5 - 5 Correct 5 answer 5 ✔C. 5 normally 5 cleaves 5 DNA 5 within 5 a 5 specific 5 sequence