BIO 171 Microbiology Final exam Summer 2025, Exams of Microbiology

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Micro Final Exam

Basic Microbiology (Portage Learning Online)

Final Exam - Requires Respondus LockDown Browser + Webcam

Due No due date Points 120 Questions 30 Time Limit 90

Minutes Requires Respondus LockDown Browser

Instructions

The Final Exam is composed of questions based upon material presented in the entire course. It will assess your level of mastery of the course content and goals. The format of the exam is similar to past module exams. You are about to begin a timed exam. Please make sure you are in a suitable, quiet place, free from distraction. Attempt History Attempt Time Score LATEST A t t e m p t (^1) 30 minutes 60 out of 120 * * Some questions not yet graded Score for this quiz: 60 out of 120 * Submitted Jul 27 at 8:57am This attempt took 30 minutes. Correct!Correct! Correct!Correct! Correct!Correct! Correct!Correct! Question 2 Not yet graded / 4^ pts Briefly explain how a Scanning Transmission Electron Holography Microscope (STEHM) works and what it is specifically used to see. Question 1 4 / 4 pts Match each of the following organelles with the correct description. Nucleus (^) Contains genomic content i Endoplasmic reticulum (^) Series of membrane-enclos Lysosomes (^) Contain hydrolytic enzymes Chloroplasts (^) Site of photosynthesis

Correct!Correct! YouYou^ AnsweredAnswered Correct Answ Correct!Correct! Correct!Correct! You You (^) AnsweredAnswered You You AnsweredAnswered You You AnsweredAnswered Question 6 4 / 4 pts A researcher is looking to solidify an agent for growth media. What polysaccharide substance would he or she use? Question 5 1 / 4 pts Match each of the following terms with the correct description. Phototroph (^) Utilize photons to generate Lithotroph (^) Acquire energy from prefor Correct Answer (^) Remove electrons from inorganic molecules Autotroph (^) Remove electrons from inor Correct Answer (^) Obtain their source of carbon from inorganic molecules Chemotroph (^) Obtain their source of carbo Correct Answer (^) Acquire energy from preformed chemicals in the environment

Correct!Correct! Question (^7) Not yet graded / 4 pts Place the following viral life cycle steps in order beginning with viral attachment (3points) and provide a description of “RELEASE” (1 point). Uncoating: Release: Replication: Attachment: Entry: New infection: Your Answer:

1. Attachment
2. Entry
3. Uncoating
4. Replication
5. Release - newly replicated virions are released from host cell into organism
6. New Infection Peptone Agar Beef Abstract Yeast

Correct!Correct! Correct!Correct! Question 10 Not yet graded / 4^ pts Question 8 4 / 4 pts Which type of bacteria appears purple/violet in color after staining? Neither Gram-positive nor Gram-negative Gram-positive Both Gram-positive and Gram-negative Gram-negative Question 9 4 / 4 pts Left untreated, three percent of cases of Streptococcal pharyngitis will progress to which of the following? Rheumatic fever Rheumatic fever occurs in approximately three percent of untreated cases of Streptococcal pharyngitis, presenting approximately 2 to 3 weeks post-infection. Laryngitis Klebsiella pneumonia Conjunctivitis

Describe TWO differences between DNA and RNA. Your Answer:

1. DNA cannot leave the nucleus, RNA can.
2. DNA must be transcribed into RNA so that RNA can be translated into proteins. Answer: Any of the following are acceptable answers: RNA contains the sugar ribose rather than deoxyribose. RNA is always single-stranded, whereas DNA is double- stranded. While RNA contains the bases adenine, guanine, and cytosine, it does not utilize the pyrimidine base thymine. Instead, RNA contains uracil (U), which still pairs with adenine (A). Correct!Correct! Question 12 4 / 4 pts Each of the following statements about respiration is true, except: It does not produce ATP It is an aerobic process It produces NADH and FADH2 in abundance The central cycle is called the Tricarboxylic Acid Cycle Answer: False; Alpha hemolysis presents as greenish-brown color colonies due to the incomplete (partial) lysis of red blood cells. Question 11 Not yet graded / 4^ pts Your Answer: False. When grown on Blood Agar Plates (BAP), microbes capable of alpha hemolysis present brownish-red color colonies due to the incomplete (partial) lysis of red blood cells. True or False. If the statement is false, change it to make it true. When grown on Blood Agar Plates (BAP), microbes capable of alpha hemolysis present as tan/ white color colonies due to the incomplete (partial) lysis of red blood cells.

You want to observe the movement of microorganisms that are present in a sample of cheek cells obtained on a patient. Briefly explain the technique that you would use and how it is performed. Your Answer:

1. We would take a swab from the cheek and smear it on a slide. We would NOT fix or dye the sample because we want to observe movement and fixing a sample kills it.
2. Place a drop of waer on the slide and place a slide cover over the sample. Depending on the magnification lens, it can be dry or oiled. If the lens says oil, we do that.
3. Use the stage clips and place the slide on the stage.
4. Adjust the magnification and the clarity of the image using the course and fine focus knobs. Answer: A wet mount stain is indicated. A small liquid culture (usually just a drop) containing a microorganism of interest is prepared, added to a slide, and then covered with a glass coverslip. The coverslip is present to both protect the objective and the specimen while also holding the microorganism in place. Wet mounts are used to observe the motility and behavior of an organism. YouYou AnsweredAnswered Correct Answ Correct!Correct! Question 16 0 / 4 pts What coronavirus-associated infection gave rise to quarantining and travel restrictions in 2012? SARS RSV COVID- ARDS er (^) MERS Question 17 4 / 4 pts Match each type of agar plate to the corresponding general characteristics of each. A multi-purpose media capable of (^) Trypticase Soy Agar growing a wide variety of microorganisms.

Correct!Correct! Correct!Correct! Correct!Correct!

Answer: The mRNA encodes only for a spike protein and not the other components of the virus. Therefore, new virions or virus particles cannot be made or replicated. Correct!Correct! Correct!Correct! Correct!Correct! Correct!Correct! Correct!Correct! Correct!Correct! Question 22 4 / 4 pts Which of the following reactions is an example of an oxidoreductase? a. A + B → A-B b. A-B + H2O → A-OH + B-H c. A— + B →A + B— d. Ab + C → A + Cb d. Question 21 4 / 4 pts Match each type of microscopy with the correct description. The simplest form of light, or optical, (^) Bright Field Microscope microscopes. Light, most often emitted from a standard halogen bulb, enters the microscope from the base (bottom) and is reflected via mirrors towards the sample. Incoming light is in the form of the (^) Fluorescence Microscope ultraviolet (UV) spectrum. Can provide detailed images of live (^) Phase Contrast Microscope cells without staining. Use of beams of electrons (rather than (^) Electron Microscope light) to increase its resolution capacity to less than 1nm—that’s 200x. Combines the usefulness of (^) Confocal Microscope fluorescence microscopy with the ability to visualize cells in 3-D This microscope reflects light off of the (^) Dark field Microscope specimen at an angle rather than light passing through the specimen.

Correct!Correct! Correct!Correct! b. a. c. Question 24 4 / 4 pts Identify the following disease based upon the physical manifestation: Gas gangrene Tetanus Hansen’s disease Anthrax Answer: Lids placed back onto the plate, they are inverted to prevent any potential contaminants from settling onto the surface of the agar and placed in an incubator set at 37°C for ~12-24 hours. Question 23 Not yet graded / 4^ pts After a microbiologist obtains a quadrant streak sample, what is the next action taken in the incubation process to obtain growth? Your Answer: Sample collected. Sample is smeared onto agar plate. Information (name, date, experiment number) is written on the bottom. Incubated at 37 degrees overnight, or 12-14 hours.

Answer: The physician should expect to receive a report of a distinct metallic green growth pattern growing on the agar. This will confirm the organism as E. coli. Question 28 Not yet graded / 4^ pts True or False. If the statement is false, change it to make it true. In addition to being a rich source of carbon and energy, Amino acids are a rich source of nitrogen and sulfur. Your Answer: True. Answer: True Correct!Correct! Correct!Correct! Question 29 4 / 4 pts Giemsa staining is used to detect each of the following, except : Chlamydia trachomatis Malaria Neisseria Gonorrhea Histoplasma Question 30 4 / 4 pts What STD is caused by Gram-negative, non-motile, rod-shaped bacteria that can cause infertility and pelvic inflammatory disease? Syphilis Leprosy Chlamydia

Gonorrhea Quiz Score: 60 out of 120