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Bean Beetle Lab Project, Lab Reports of Zoology

Bean Beetles is sexually dimorphic and also its life cycle is explained.

Typology: Lab Reports

2020/2021

Uploaded on 05/11/2021

koss
koss 🇺🇸

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Bean Beetle Lab Project
Introduction
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Bean Beetle Lab Project

Introduction

Bean Beetles (Callosobruchus maculatus)

  • Tropical and subtropical pests that originated in west Africa and are widespread throughout much of Africa and Asia
  • Often sold as food for pet reptiles and amphibians
  • Sexually dimorphic Photos from Beck and Blumer 2011, male left, female right.

Our Scientific Question:

  • Are there differences between populations of beetles that have been raised on different types of source beans? - Visual differences? - Behavioral differences? - Genetic differences? Why should we care? Figure from Beck and Blumer 2011

Lab 1: Looking for Visual Differences

  • We have 4 beetle populations:
    • JF – obtained from Josh’s Frogs, raised on black-eyed peas
    • WM – obtained from Ward’s Science, raised on mung beans
    • WB – obtained from Ward’s Science, raised on black-eyed peas
    • WA – obtained from Ward’s Science, raised on adzuki beans
  • We will use dissecting (stereo) microscopes and compound light microscopes to look for visual differences between beetles from the different populations.

Lab 1:

Objectives:

  • Familiarize yourself with microscope technique (compound and dissecting microscopes)
  • Observe characteristics of male and female bean beetles from each of 4 populations
  • Determine whether there are visual differences between beetles from different populations Background section should include:
  • Diagrams or drawings of a compound light microscope and a dissecting (also called stereo) microscope (cite sources!)
  • Information about when each type of microscope should be used vs. the other type
  • Descriptions of basic beetle anatomical structures. Focus on external anatomy, not internal.
  • How to tell male and female bean beetles ( Callosobruchus maculatus) apart Materials and Methods should include:
  • Steps for making a wet mount slide and viewing it under a compound light microscope at high magnification
  • Steps for viewing a specimen under a dissecting microscope (also called a stereo microscope)

Starting your Lab Notebook

2 1/14/ Signature 1/16/09 3 Signature DETAILS:

  • 1 st pages – contact info for group, then table of contents
  • Use PEN only for lab notebooks
  • Errors – no white out or scribbles, draw a single line through the error.
  • ALL pages should be numbered in the UPPER OUTSIDE corner of the page
  • The DATE should be written at the very top of each page adjacent to the page number
  • Your SIGNATURE should be included at the bottom of each page
  • A component of your grade is how well you follow these instructions. Following them = free points.
  • Write on the lines – no squishing.
  • Use clear headings for each section – use a separate line for the heading of each section
  • Each week’s lab should start on a new page.

Example Background: credit Rachelle Mendoza

Example Background: credit Micaela Gray

Reminders:

Which of these are acceptable? What will happen

if you plagiarize?

  • Copying a few phrases directly from a source and citing that source
  • Copying a few phrases directly from a source, putting the copied parts in parentheses, and citing the source
  • Copying multiple paragraphs directly from a source, putting the copied parts in parentheses, and citing the source
  • Paraphrasing ideas you found on a reputable website in your own words and citing the website
  • Paraphrasing ideas you found on a reputable website in your own words without citing the website
  • Taping in a photo taken by your group member and crediting them by name underneath the photo

Sources: Beck, C.W. and Blumer, L.S. (2011). A Handbook on Bean Beetle, Callosobruchus maculatus. National Science Foundation: Pp. 1-10. www.beanbeetles.org Lab notebook examples – thanks to students Rachelle Mendoza and Micaela Gray from Fall 2016 Biol 160 for sharing examples from their work