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Bean Beetle Lab Project
Introduction
Bean Beetles (Callosobruchus maculatus)
- Tropical and subtropical pests that originated in west Africa and are widespread throughout much of Africa and Asia
- Often sold as food for pet reptiles and amphibians
- Sexually dimorphic Photos from Beck and Blumer 2011, male left, female right.
Our Scientific Question:
- Are there differences between populations of beetles that have been raised on different types of source beans? - Visual differences? - Behavioral differences? - Genetic differences? Why should we care? Figure from Beck and Blumer 2011
Lab 1: Looking for Visual Differences
- We have 4 beetle populations:
- JF – obtained from Josh’s Frogs, raised on black-eyed peas
- WM – obtained from Ward’s Science, raised on mung beans
- WB – obtained from Ward’s Science, raised on black-eyed peas
- WA – obtained from Ward’s Science, raised on adzuki beans
- We will use dissecting (stereo) microscopes and compound light microscopes to look for visual differences between beetles from the different populations.
Lab 1:
Objectives:
- Familiarize yourself with microscope technique (compound and dissecting microscopes)
- Observe characteristics of male and female bean beetles from each of 4 populations
- Determine whether there are visual differences between beetles from different populations Background section should include:
- Diagrams or drawings of a compound light microscope and a dissecting (also called stereo) microscope (cite sources!)
- Information about when each type of microscope should be used vs. the other type
- Descriptions of basic beetle anatomical structures. Focus on external anatomy, not internal.
- How to tell male and female bean beetles ( Callosobruchus maculatus) apart Materials and Methods should include:
- Steps for making a wet mount slide and viewing it under a compound light microscope at high magnification
- Steps for viewing a specimen under a dissecting microscope (also called a stereo microscope)
Starting your Lab Notebook
2 1/14/ Signature 1/16/09 3 Signature DETAILS:
- 1 st pages – contact info for group, then table of contents
- Use PEN only for lab notebooks
- Errors – no white out or scribbles, draw a single line through the error.
- ALL pages should be numbered in the UPPER OUTSIDE corner of the page
- The DATE should be written at the very top of each page adjacent to the page number
- Your SIGNATURE should be included at the bottom of each page
- A component of your grade is how well you follow these instructions. Following them = free points.
- Write on the lines – no squishing.
- Use clear headings for each section – use a separate line for the heading of each section
- Each week’s lab should start on a new page.
Example Background: credit Rachelle Mendoza
Example Background: credit Micaela Gray
Reminders:
Which of these are acceptable? What will happen
if you plagiarize?
- Copying a few phrases directly from a source and citing that source
- Copying a few phrases directly from a source, putting the copied parts in parentheses, and citing the source
- Copying multiple paragraphs directly from a source, putting the copied parts in parentheses, and citing the source
- Paraphrasing ideas you found on a reputable website in your own words and citing the website
- Paraphrasing ideas you found on a reputable website in your own words without citing the website
- Taping in a photo taken by your group member and crediting them by name underneath the photo
Sources: Beck, C.W. and Blumer, L.S. (2011). A Handbook on Bean Beetle, Callosobruchus maculatus. National Science Foundation: Pp. 1-10. www.beanbeetles.org Lab notebook examples – thanks to students Rachelle Mendoza and Micaela Gray from Fall 2016 Biol 160 for sharing examples from their work