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BCHM 270 – biotechnologies QUESTIONS WITH ANSWERS, Exams of Biochemistry

Galen Health Institute - Tampa Bay Biochemistry

BCHM 270 – biotechnologies QUESTIONS WITH ANSWERS

Typology: Exams

2024/2025

Available from 07/07/2025

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BCHM |\270 |\– |\biotechnologies |\QUESTIONS |\

WITH |\ANSWERS

restriction |\endonucleases |\- |\CORRECT |\ANSWERS |\✔✔- |\found |\in |\

double |\stranded |\dna |\at |\specific |\sequences |\

- |\this |\cleavage |\results |\in |\the |\production |\of |\dna |\fragments |\known |\as

|\restriction |\fragments

why |\do |\bacteria |\use |\restriction |\endonucleases? |\- |\CORRECT |\

why |\do |\scientists |\use |\restriction |\endonucleases? |\- |\CORRECT |\

✔✔first: |\genus |\of |\bacterium

next |\two |\letters: |\species

e.g. |\HaeIII

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BCHM |\ 270 |\– |\biotechnologies |\QUESTIONS |\

WITH |\ANSWERS

|\this |\cleavage |\results |\in |\the |\production |\of |\dna |\fragments |\known |\as |\restriction |\fragments why |\do |\bacteria |\use |\restriction |\endonucleases? |- |\CORRECT |
ANSWERS |\✔✔these |\enzymes |\are |\used |\to |\recognize |\and |\identify |
foreign |\dna |\from |\invading |\species |(e.g. |\viruses) why |\do |\scientists |\use |\restriction |\endonucleases? |- |\CORRECT |
ANSWERS |\✔✔used |\to |\clone |\dna |+ |\combine |\dna |\sequences |\together restruction |\endonucleas |\nomenclature: |- |\CORRECT |\ANSWERS |
✔✔first: |\genus |\of |\bacterium next |\two |\letters: |\species number: |\order |\in |\which |\enzyme |\was |\discovered e.g. |\HaeIII

gel |\electrophoresis |\purpose |- |\CORRECT |\ANSWERS |\✔✔to |\separate |
dna |\segments |\based |\on |\charge usually |\charge |\corresponds |\to |\size method |\of |\gel |\electrophoresis |- |\CORRECT |\ANSWERS |\✔✔separated |
on |\a |\gel |(polyacrimde |\or |\agarose) |+ |\application |\of |\electrical |\field: |
the |\smaller |\the |\molecule, |\the |\farther |\it'll |\travel |\along |\the |\gel dna |\cloning |- |\purpose? |- |\CORRECT |\ANSWERS |\✔✔to |\amplify |\a |\DNA |
sequence |\of |\interest dna |\cloning |- |\method |- |\CORRECT |\ANSWERS |\✔✔1. |\dna |\is |\cleaved |
using |\restriction |\endonucleases

|\resulting |\fragments |\are |\joined |\to |\a |\dna |\vector, |\using |\dna |\ligase; |
this |\produces |\a |\hybrid |\molecule |\
|\hybrid |\molecule |\is |\introduced |\into |\bacterial |\cell; |\bacterial |\cell |
divides, |\effectively |\producing |\a |\copy |\of |\the |\cloned |\dna what |\are |\dna |\probes? |- |\CORRECT |\ANSWERS |\✔✔- |\single |\stranded |
dna |\molecules |\that |\are |\labelled |\with |\a |\radioactive |\isotope |\or |
flurorescent |\dye

what's |\an |\allele-specific |\oligonucleotide? |- |\CORRECT |\ANSWERS |
✔✔oligonucleotides |\can |\be |\designed |\to |\bind |\to |\only |\one |\version |\of |\an |\allele; |\i.e. |\can |\be |\used |\to |\identify |\a |\specific |\polymorphism/mutation describe |\the |\technique |\of |\ASO |- |\CORRECT |\ANSWERS |\✔✔dna |
samples |\are |\mixed |\with |\ASO; |\aso |\will |\identify |\gene |\of |\interest; |\the |
probes |\will |\attach |\if |\dna |\sequence |\of |\interest |\is |\present |\and |\can |\be |\visualized define |\blotting |- |\CORRECT |\ANSWERS |\✔✔trasnfrer |\of |\macromolecules |\on |\a |\solid-phase |\membranous |\support what |\are |\dna/rna |\molecules |\separated |\via |\gel |\electrophoresis |
blotted |\on? |- |\CORRECT |\ANSWERS |\✔✔nylon |\or |\nitrocellulose |
membrane purpose |\of |\southern |\blotting? |- |\CORRECT |\ANSWERS |\✔✔to |\detect |
specfic |\dna |\sequences |\in |\dna |\samples; |\as |\well |\as |\the |
concentration/intensity |\of |\each |\dna |\fragement |\of |\interest northern/southern |\blotting |\technique |- |\outline. |- |\CORRECT |\ANSWERS |\✔✔1. |\gel |\electrophoresis |- |\dna/rna |\is |\digested |\via |\restriction |
endonucleases |\and |\separated |\by |\size |\using |\gel |\electrophoresis |\

|\separated |\fragements |\are |\trasnferred |\to |\a |\nylon/PVDF |\membrane |+ |\washed |\with |\blocking |\solution |\that |\woll |\reduce |\non-specific |
binding |\
|\membrane |\is |\washed |\with |\a |\radiolabelled |\probe |(or |\fluroscent |
dye) |- |\probe |\will |\bind |\to |\dna |\sequences |\of |\interest
|\target |\dna |\sequences |\are |\detected |\by |\the |\use |\of |\exposure |\film |
(radiolabelled |\probes) |\or |\fluroscent |\detectors; |\intensity |\of |\the |\bands |\produced |\is |\proportional |\to |\the |\concentration |\of |\the |\target |\dna |
sequence |\found, PCR |- |\purpose? |- |\CORRECT |\ANSWERS |\✔✔to |\amplify |\a |\selected |\dna |
sequences; |\millions |\and |\billions |\of |\copies |\of |\a |\single |\sequence |
created |\in |\just |\a |\few |\hours what |\components |\are |\required |\for |\PCR? |- |\CORRECT |\ANSWERS |
✔✔DNA |\primer, |\dNTP |\nucleotides, |\Taq |\polymerase, |\magnesium, |
DNA |\sequence |\of |\interest. what |\machine |\is |\used |\for |\PCR? |- |\CORRECT |\ANSWERS |
✔✔thermocycler |(can |\rapidly |\change |\temp.) outline |\PCR |\method. |- |\CORRECT |\ANSWERS |\✔✔1. |\DNA |\sequence |\of |
interest, |\dNTP |\nucleotides, |\Taq |\polymerase, |\DNA |\primers |\are |\mixed |\in |\a |\tube

BCHM 270 – biotechnologies QUESTIONS WITH ANSWERS, Exams of Biochemistry

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Download BCHM 270 – biotechnologies QUESTIONS WITH ANSWERS and more Exams Biochemistry in PDF only on Docsity!

BCHM |\ 270 |\– |\biotechnologies |\QUESTIONS |\

WITH |\ANSWERS