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BACTERIAL GROWTH CALCULATION, Study notes of Biotechnology

The process of counting bacterial growth by counting colonies on an agar plate. It also provides formulas for calculating the growth rate constant and mean generation time. The exponential phase and biomass calculation are also discussed. examples and graphs to illustrate the concepts. The text is machine-translated by Google and may contain errors.

Typology: Study notes

2021/2022

Available from 12/16/2022

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by counting the number of colonies growing on the agar plate, we can also count
BACTERIAL GROWTH CALCULATION
time is not possible from point 0 directly to point 4 days, if we count
all the results of the numbers that we get when we count, of course, follow every calculation
the population of bacteria or microorganisms doubles from 100 to 200, then
MEETING 7
by calculating its dry biomass.
generation uses some of the formulas shown:
(note the exponential phase and the green line) for example from a 6 hour time interval
for example how many after several generations, the speed of growth and time
will continue to follow.
(though not as smooth as the example in the curve above), because in phase this is the exponential quantity
using a counting chamber or petrovaoser, counting the number of cells indirectly
how to count bacteria directly count cells both alive and dead
we follow the third hour then later we will know where the exponential point is
the amount or know the growth by weighing the cell biomass, for example
the calculation is valid when the bacteria are in the exponential phase.
Machine Translated by Google
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by counting the number of colonies growing on the agar plate, we can also count

BACTERIAL GROWTH CALCULATION

time is not possible from point 0 directly to point 4 days, if we count all the results of the numbers that we get when we count, of course, follow every calculation the population of bacteria or microorganisms doubles from 100 to 200, then

MEETING 7

by calculating its dry biomass. generation uses some of the formulas shown: (note the exponential phase and the green line) for example from a 6 hour time interval for example how many after several generations, the speed of growth and time will continue to follow. (though not as smooth as the example in the curve above), because in phase this is the exponential quantity using a counting chamber or petrovaoser, counting the number of cells indirectly how to count bacteria directly count cells both alive and dead we follow the third hour then later we will know where the exponential point is the amount or know the growth by weighing the cell biomass, for example the calculation is valid when the bacteria are in the exponential phase.

shows its generation so the example is in the fifth generation be 2. below is the result of calculations from research that has been collected in in the description it was explained that the super script numbers above the number of cells is 32, then the power of 2, number 2 is because some bacteria divide binary or fold book, which explains the number of cells from generation to generation

then the bacterial population formula is × ( is the number of bacteria minutes later, if the N0 is 1?) so from the formula above to find the population is: when at time 0 that is 1) then from the first generation the number is 2 if it is logged (log it so the numbers don't get too big) 3 (THIS FORMULA CAN BE USED BY QUESTION what is the population at 120 the superscript rank is the generation, so for example in the fifth generation then bacteria has divided five times (the number of cells is 32).

mean growth rate constant (K), so the number of generations per unit time is expressed as hourly generation. ,K the growth rate constant is also the number of generations then we can calculate the growth rate constant: timer then the formula:

PROBLEMS EXAMPLE :