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Answer Key to Past Exam 2 - Principles of Biochemistry | BCH 4013, Exams of Biology

Material Type: Exam; Professor: Li; Class: Principles of Biochemistry; Subject: Biochemistry; University: Mississippi State University;

Typology: Exams

2011/2012

Uploaded on 02/27/2012

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BCH 4013/6013 Exam 2 (10/07/2010)
There are 50 questions worth
0.4 points each
(total 20 points)
1. If the G'° of the reaction A B is –30 kJ/mol, under standard conditions, the reaction:
A) is at equilibrium.
B) will proceed spontaneously from right to left.
C) will not occur spontaneously.
D) will proceed spontaneously from left to right.
2. The conversion of 1 mol of glucose to 2 mol of pyruvate by the glycolytic pathway results in a
NET formation of:
A) 1 mol of NAD
+
and 2 mol of ATP
B) 1 mol of NADH and 1 mol of ATP
C) 2 mol of NAD
+
and 4 mol of ATP
D) 2 mol of NADH and 2 mol of ATP
3. Gluconeogenesis is the biosynthesis of glucose from simpler, noncarbohydrate precursors such
as:
A) pyruvate
B) Acetate
C) ATP
D) ADP
4. The pentose phosphate pathway produces:
A) thiamine pyrophosphate
B) ribose 5-phosphate and NADPH
C) biotin
D) ethanol
5. The storage form of carbohydrates in animals is:
A) glucose
B) amylopectin
C) lactose
D) glycogen
6. The glycogen-branching enzyme catalyzes:
A) formation of 1-4 linkages during glycogen degradation
B) formation of 1-4 linkages during glycogen synthesis
C) formation of 1-6 linkages during glycogen degradation
D) formation of 1-6 linkages during glycogen synthesis
7. The conversion of 2 mol of pyruvate to 1 mol of glucose by gluconeogenesis requires:
A) 2 mol of ATP
B) 4 mol of ATP and 2 mol of GTP
C) 2 mol of GTP
D) none of the above
8. Brewing beer is a process of:
A) ethanol fermentation of carbohydrate
B) lactic acid fermentation of carbohydrate
C) all of the above
D) none of the above.
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BCH 4013/6013 Exam 2 (10/07/2010)

There are 50 questions worth 0.4 points each (total 20 points)

  1. If the ∆ G' ° of the reaction A → B is –30 kJ/mol, under standard conditions, the reaction: A) is at equilibrium. B) will proceed spontaneously from right to left. C) will not occur spontaneously. D) will proceed spontaneously from left to right.
  2. The conversion of 1 mol of glucose to 2 mol of pyruvate by the glycolytic pathway results in a NET formation of: A) 1 mol of NAD

and 2 mol of ATP B) 1 mol of NADH and 1 mol of ATP C) 2 mol of NAD

and 4 mol of ATP D) 2 mol of NADH and 2 mol of ATP

  1. Gluconeogenesis is the biosynthesis of glucose from simpler, noncarbohydrate precursors such as: A) pyruvate B) Acetate C) ATP D) ADP
  2. The pentose phosphate pathway produces: A) thiamine pyrophosphate B) ribose 5-phosphate and NADPH C) biotin D) ethanol
  3. The storage form of carbohydrates in animals is: A) glucose B) amylopectin C) lactose D) glycogen
  4. The glycogen-branching enzyme catalyzes: A) formation of 1-4 linkages during glycogen degradation B) formation of 1-4 linkages during glycogen synthesis C) formation of 1-6 linkages during glycogen degradation D) formation of 1-6 linkages during glycogen synthesis
  5. The conversion of 2 mol of pyruvate to 1 mol of glucose by gluconeogenesis requires: A) 2 mol of ATP B) 4 mol of ATP and 2 mol of GTP C) 2 mol of GTP D) none of the above
  6. Brewing beer is a process of: A) ethanol fermentation of carbohydrate B) lactic acid fermentation of carbohydrate C) all of the above D) none of the above.
  1. What type of linkage(s) is/are between the glucose units in glycogen? A) α-1, B) beta-1, C) α-1,4 and α-l, D) beta-l,4 and beta-l,
  2. The reactions of glycolysis occur in_______: A) mitochondria B) cytosol C) nucleus D) endoplasmic reticulum
  3. The ________ is the site of most gluconeogenesis in mammals. A) liver B) pancreas C) heart D) small intestine
  4. Glycosidic bond is the primary structural linkage in A) proteins B) polysaccharides C) lipids D) insulin
  5. Which substance is a product of glycolysis, a precursor of gluconeogenesis? A) glucose B) pyruvate C) ATP D) coenzyme A
  6. Consider the reaction A → B, if the free energy of B is greater than the free energy of A, then ∆G will be: A) negative and the reaction is said to be exergonic B) positive and the reaction is said to be endergonic C) negative and the reaction is said to be exothermic D) positive and the reaction is said to be endothermic
  7. _________ can regulate many metabolic enzymes. A) methylation B) acetylation C) phosphorylation D) deacetylation
  8. Phosphorylation can be either inactive or active enzymes. This is a key element in the regulation of glycogen metabolism. Phosphorylation of glycogen phosphorylase ______ it; while phosphorylation of glycogen synthase ______ it. A) activates; activates B) activates; inactivates C) inactivates; inactivates D) inactivates; activates
  1. Which of the following statements about the chemiosmotic theory is FALSE? A) Electron transfer in mitochondria is accompanied by proton transfer across the membrane B) The inner mitochondrial membrane is impermeable to protons C) Oxidative phosphorylation can occur in membrane-free preparations D) The proton gradient provides the energy for ATP synthesis by ATP synthase
  2. A recently discovered bacterium carries out ATP synthesis coupled to the flow of electrons through a chain of carriers to some electron acceptor. The components of its electron transfer chain differ from those found in mitochondria; they are listed below with their standard reduction potentials ( E' °).

Oxidant Reductant E' °(V) NAD

+ NADH –0. flavoprotein b ( FP b ) (oxidized) flavoprotein b (reduced) –0. cyt c (Fe

3+ ) cyt c (Fe

2+ ) +0. Fe-S protein (oxidized) Fe-S protein (reduced) +0. flavoprotein a (FP a ) (oxidized) flavoprotein a (reduced) +0.

The electron carriers are most likely to act in carrying electrons in the order: A) NAD+^ → FP b → cyt c → FP a → Fe-S B) FP b → cyt c → FP a → Fe-S→ NAD+ C) FP b → NAD+^ → FP a → cyt c → Fe-S D) FP b → NAD+^ → cyt c → FP a → Fe-S

  1. In the mitochondria NADH and FADH 2 are essentially oxidized by _______ since it is the terminal electron acceptor. A) CO 2 B) H 2 O 2 C) O 2 D) H 2 O

28. Anything that makes the mitochondrial membrane leaky to proton stops ATP synthesis in the

mitochondria, because A) it prevents formation of a proton gradient across the membrane B) it stimulates formation of a proton gradient across the membrane C) it can bind to NADH D) it can bind to NAD+

  1. Cyanide and carbon monoxide are lethal poisons because they can block in mitochondria. A) electron transfer B) ATP transfer C) ADP transfer D) AMP transfer
  2. Chloroplasts have membranes which contain ATP synthase similar to: A) Nuclei B) Golgi apparatus C) Peroxisomes D) Mitochondria
  1. During photophosphorylation in plants, electrons flow through a series of carriers in the chloroplast. The ultimate electron donor and acceptor are ___________, respectively: A) NADP+^ and H 2 O B) H 2 O and NADP+ C) O 2 and H 2 O D) H 2 O and O 2
  2. The light reactions in photosynthetic higher plants: A) do not require chlorophyll B) produce O 2 , ATP, and NADPH C) produce CO 2 D) serve to produce light so that plants can see underground
  3. What is the source of carbons for the Calvin cycle? A) glucose B) carbon dioxide C) glycogen D) none of the above
  4. In which part of the chloroplast do the light-dependent reactions of photosynthesis take place? A) in the thylakoid lumen B) in the thylakoid membrane C) in the stroma D) in the outer membrane
  5. The process of photosynthetic carbon assimilation (fixation) is also called A) photorespiration B) the glycolate pathway C) the pentose phosphate pathway D) the Calvin cycle
  6. The light-dependent and carbon-assimilation reactions of photosynthesis are linked in that A) the light-dependent reactions generate the chemical energy needed for carbon- assimilation B) the oxidation of carbohydrates from the carbon-assimilation reactions drives the synthesis of ATP in the light-dependent reactions C) carbon dioxide generated in the light-driven reactions is converted to triose phosphates in the carbon-assimilation reactions D) NADPH generated in the carbon-assimilation reactions drives the synthesis of ATP in the light-dependent reactions
  7. The major pigment involved in photosynthesis is A) cytochrome C B) chlorophyll C) cryptochrome D) phytochrome
  8. The compound that condenses with CO 2 in the first reaction of Calvin cycle is:

A) 3-phosphoglycerate B) sedoheptulose 1,7-bisphosphate. C) ribulose 1,5-bisphosphate D) fructose 6-phosphate

  1. Which of the following is a catabolic pathway? A) Glycolysis B) Gluconeogenesis C) all of the above D) none of the above
  2. Under standard conditions, if the equilibrium constant (K′eq) of a reaction is 1, then one would expect the ∆G′° to be: A) negative and the reaction proceeds forward B) zero and the reaction is at equilibrium C) positive and the reaction proceeds in reverse D) there is no relationship between these parameters
  3. ATP synthase is located in the ________ of the mitochondrion. A) outer membrane B) inner membrane C) matrix D) intermembrane space
  4. The term 'rubisco' is frequently used in connection with photosynthesis. Rubisco is: A) An important enzyme in the Calvin cycle B) A cereal killer C) A breakfast cereal
  5. How many CO 2 's are produced from the complete oxidation of 1 molecule of pyruvate via the citric acid cycle? A) 1 B) 2 C) 3 D) 4

Answer Key to Exam 2

  1. D
  2. D
  3. A
  4. B
  5. D
  6. D
  7. B
  8. A
  9. C
  10. B
  11. A
  12. B
  13. B
  14. B
  15. C
  16. B
  17. D
  18. A
  19. A
  20. A
  21. C
  22. C
  23. B
  24. B
  25. C
  26. D
  27. C
  28. A
  29. A
  30. D
  31. B
  32. B
  33. B
  34. B
  35. D
  36. A
  37. B
  38. C
  39. C
  40. D
  41. C
  42. B
  43. D
  44. C
  45. C
  46. A
  47. B
  48. B
  49. A
  50. C