Dr. Susan F. Hornbuckle
Clayton State University
CHEM 4202
Chapter 14 and 15 Objectives
1. Be able to define: metabolism, anabolism, catabolism, glycolysis, pay-off phase,
preparatory phase, and metabolites.
2. Know where in the cell that glycolysis occurs.
3. Be able to draw the glycolysis pathway including the structures of all the glycolytic
intermediates and the names of all the enzymes, coenzymes, reactants and product
involved in the pathway.
4. Be able to determine the number of moles of ATP produced by the glycolysis
pathway under aerobic conditions starting with 1 mole of glucose or at any other
point in the pathway in a liver, kidney, heart, brain, or skeletal muscle cell.
5. Be able to follow an isotopically labeled carbon in glucose through the glycolysis
pathway.
6. Be able to write the overall balanced chemical equation for glycolysis under aerobic
conditions, anaerobic conditions, and fermentation conditions.
7. Be able to explain the fate of pyruvate under aerobic, anaerobic, and fermentation
conditions (including chemical equations).
8. Be able to explain the purpose of the formation of lactate under anaerobic conditions.
9. Be able to identify the regulatory enzymes in the glycolysis pathway and list their
inhibitors and activators.
10. Be able to explain the three important functions of the phosphoryl groups of the nine
phosphorylated intermediates in the glycolytic intermediates.
11. Be able to explain the concept of channeling. Give three example of channeling in
the catabolism of glucose.
12. Be able to describe the break down of glycogen (via glycogen phosphorylase and
debranching enzyme). At what point does the product of this pathway enter into
glycolysis?
13. Be able to describe the simultaneous regulation of the gluconeogenesis pathway and
the glycolysis pathway at enzymes PFK-1 and FBPase-1. How does the hormone
glucagon effect the regulation of these pathways?
14. Be able to explain the different reactions catalyzed by PFK-1 and PFK-2.
15. Know which catabolic step is shut down if a person has a Vitamin B1 deficiency.
What is the name of this medical condition?